FastQCFastQC Report
Wed 25 May 2016
SRR1779952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67549
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3415150.55737316614606No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG45756.772861182252883No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6220.9208130394232334No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT4920.7283601533701461No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG4840.7165168988438023No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG1420.21021776784260313No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG970.14359946113191904No Hit
TATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGT810.11991295207923137No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCATGTGTAGATCTCGGTG690.10214807028971562No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGT150.002215818370.046
TCTGGCG301.0097392E-770.011
AATTATA150.002215818370.01
ATCGGTG150.002215818370.044
CCATCCC207.874763E-570.017
GCGCTCT150.002215818370.027
CGTTGTA150.002215818370.047
AAACGTT207.874763E-570.044
GACCAGC150.002215818370.011
GTCTGGC352.9338844E-760.010
TGGCGCT352.9338844E-760.013
GCGTCTG308.29531E-658.3333328
AACGTTG252.3748938E-455.99999645
GCGCTGC252.3748938E-455.99999615
CGAATCA252.3748938E-455.99999656
CGCGTCT252.3748938E-455.9999967
CCGAATC252.3748938E-455.99999655
AAGGGGG653.6379788E-1253.84615370
GTAGATA200.006923864652.538
CCAAACC200.006923864652.522
Produced by FastQC (version 0.11.5)