SRR1779952_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779952_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67549
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT	22147	32.786569749367125	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8159	12.078639210054924	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG	2618	3.8757050437460214	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC	1997	2.956372411138581	No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT	1118	1.6550948200565514	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT	791	1.1710017912922472	TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC	500	0.74020340789649	No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC	367	0.5433093013960236	No Hit
ATTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT	286	0.42339634931679226	No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT	208	0.30792461768493984	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT	199	0.29460095634280303	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG	177	0.26203200639535745	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC	173	0.25611037913218554	No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC	148	0.21910020873736102	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT	136	0.20133532694784528	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC	132	0.19541369968467337	No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT	105	0.1554427156582629	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT	92	0.13619742705295415	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT	89	0.13175620660557522	No Hit
CAAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT	85	0.1258345793424033	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG	83	0.12287376571081733	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT	75	0.11103051118447349	No Hit
CACTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC	73	0.10806969755288752	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCT	15	0.0022116282	70.033356	34
CCACTGC	15	0.0022181445	69.98148	8
CATGGCG	15	0.0022181445	69.98148	2
CGTCTAC	15	0.0022181445	69.98148	48
GTCTACT	20	7.885089E-5	69.981476	49
AGGGGGG	2855	0.0	63.77819	70
GTAAGCT	35	2.9392686E-7	59.98413	32
CTTAAAC	35	2.9392686E-7	59.98413	1
ATTATAC	65	0.0	59.2151	1
TAAGCTC	30	8.308327E-6	58.3179	33
CACTTAT	30	8.308327E-6	58.3179	1
CTATACA	55	3.45608E-11	57.257572	1
CATATAC	185	0.0	56.74174	1
CGTATGA	50	9.549694E-10	56.026688	41
TAAACAC	50	9.604264E-10	55.985188	3
CCGTCTA	25	2.378E-4	55.985188	47
AATATAC	50	9.604264E-10	55.985188	1
AAGGGGG	3265	0.0	55.447544	69
ATATACA	240	0.0	55.402004	2
GTATGAC	45	2.6429916E-8	54.470387	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content