FastQCFastQC Report
Wed 25 May 2016
SRR1779951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214457
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2208410.297635423418214No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75003.4972045678154595No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC25721.199308019789515No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG22641.0556894855378933No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT6940.32360799600852386No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT6440.30029329888975415TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC4420.2061019225299244No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2710.12636565838373193No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT2200.10258466732258681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG24900.060.58071570
AACTCGT352.9650255E-759.998437
TAACTCG352.9650255E-759.998436
AGCCGTC601.8189894E-1258.3317845
AATCTCG308.358287E-658.3317836
CTTTATA653.6379788E-1253.844722
CTGCTGA200.006945099752.498636
TGGACAC200.006945099752.498665
CTGCACG200.006945099752.49869
CTCGCTG200.006945099752.498641
TTAAACG200.006945099752.498614
TATGTCG200.006945099752.498632
GCCGTCT29600.051.7891646
CCGTCTT29500.051.72743647
CGTCTTC28650.051.6740248
TCTTCTG27950.051.2150950
CTTGAAA30100.050.92887557
CGTATGC32050.050.77867541
GTCTTCT28400.050.6500649
AAGGGGG29800.050.61945369