FastQCFastQC Report
Wed 25 May 2016
SRR1779950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348983
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG336939.654625010387326No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG39501.131860291188969No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC7010.2008693833223968No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG5930.16992231713292624No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT4330.12407481166704394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA150.002223404170.0015719
ACGTCGA150.002224668269.9915410
CGTATGT200.006947380552.50118351
ACGTATG200.006947380552.50118350
GTTCTAT305.877244E-446.6610261
GGTCTAA350.001255312939.995171
GGTCCCA459.195271E-538.884191
TCGCCGT6850.037.81107352
TGGTCGC7200.037.431449
GCCGTAT6900.037.02982354
CGGTGGT7500.036.40081846
TCGGTGG7550.036.15975645
CGTATCA7250.035.72494556
CGCCGTA7250.035.72494553
TCTCGGT7450.035.2356943
TAGATCT7450.035.2356939
ACCCGAT400.00241686535.0007927
TACCGAC400.00241686535.0007967
AGATCTC7400.035.00078640
GATCATA400.002418564934.9957731