FastQCFastQC Report
Wed 25 May 2016
SRR1779950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348983
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT219426.287412280827433No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73292.1001022972465706No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC28710.8226761762034254No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG23240.6659350168919403No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT8910.255313296063132RNA PCR Primer, Index 19 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT7330.21003888441557325No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC4060.11633804511967633No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG150.002224107769.9959914
AGGGGGG28300.060.8445770
AAGGGGG32300.052.65952369
ATCGTAT407.472154E-752.4969939
CTCGACA200.00694957652.4969938
CCGTCTT33850.050.76518647
GCCGTCT34200.050.45032546
CGTCTTC34150.050.42170748
TATGCCG34250.050.2744943
AGCCGTC352.0851674E-549.99713545
ATGCCGT34300.049.99713544
ATCTCGT34650.049.9971337
CGTATGC34800.049.882241
TCGTATG34950.049.8683940
CTCGTAT34650.049.79512439
TGCCGTC34450.049.77943845
GTATGCC34700.049.72337342
CTTGAAA34850.049.6097857
TCTCGTA35000.049.59715738
AATCTCG34800.049.5804936