FastQCFastQC Report
Wed 25 May 2016
SRR1779949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235141
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3951016.802684346838706No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG45281.9256531187670376No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6160.26197047728809525No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT5070.2156153116640654No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG5040.21433948141753248No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGTG150.002222697370.000011
TTGCGTT207.9155245E-570.0000138
GCGGAAT150.002222697370.0000156
ATTTGCG207.9155245E-570.0000136
CGAGTTC200.00694519452.50000460
TTGTACT200.00694519452.5000044
ATGTACA200.00694519452.50000445
TCAATCC200.00694519452.50000440
CCGAGTT200.00694519452.50000459
ATAAGTT200.00694519452.50000422
CGTACGC200.00694519452.50000454
GGCATCC200.00694519452.50000436
CGATGCC200.00694519452.50000416
TGCACAA200.00694519452.50000433
TCCGGGT200.00694519452.5000048
GCCGTAT7200.049.58333654
TCGCCGT7250.049.2413852
CTCGGTG7050.049.14893344
AGATCTC7100.048.8028240
CGTATCA7250.048.7586256
Produced by FastQC (version 0.11.5)