FastQCFastQC Report
Wed 25 May 2016
SRR1779949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235141
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2602811.069103218919713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78683.346077459907035No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG28661.2188431621877938No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC25631.0899843072879676No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT11390.48439021693366957No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT8490.36105995976881955No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5540.23560331885974797No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3830.1628809948073709No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT2810.11950276642525123No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2490.1058939104622333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGA150.00222307369.99702566
CATCTCG150.00222307369.99702536
TCGGATC150.00222307369.99702534
TTGCGTC150.00222307369.99702511
CCGACTT301.0210533E-769.99702547
ATGGTCA150.00222307369.99702538
ATCGGAT150.00222307369.99702533
TGCCGAC301.0210533E-769.99702545
CGTCTTA207.917194E-569.9970248
AATATCG550.063.6336636
ATATCGT501.4551915E-1162.99732237
CGAATGC401.0626536E-861.2473941
AGGGGGG32500.061.1666370
TCTACGT308.3615705E-658.33085335
CTACGTC308.3615705E-658.33085336
AATTCAC308.3615705E-658.33085358
GCCGTCA601.8189894E-1258.33085346
GCCGACT452.6921043E-854.4421346
TCTGAAG200.006940521852.50893421
ATCGTAT608.367351E-1152.4977739
Produced by FastQC (version 0.11.5)