FastQCFastQC Report
Wed 25 May 2016
SRR1779948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54245
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3198458.962116324085166No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG39577.294681537468891No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT4530.8351000092174394No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC4400.8111346667895659No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG3860.7115863213199373No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG1240.2285924970043322No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG780.14379205456724123No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT207.860762E-570.000011
CAATAAG150.00221345370.044
TGAACCA150.00221345370.047
AAAGGGG308.2733095E-658.33333270
TACTCCA252.3706973E-455.99999628
AAAAAGG2700.054.44444770
ATGTGTA200.00691652952.50000434
AAAATAT200.00691652952.50000415
CATGTGT200.00691652952.50000433
ATCCTTG200.00691652952.50000430
CTATCCT200.00691652952.50000434
TCTCGGT6700.048.5820943
GATCTCG6850.048.54014641
AGATCTC6850.048.54014640
ATCTCGG6800.048.38235542
CTTGTGT6600.048.25757633
TCGGTGG6750.048.2222245
CTCGGTG6750.048.2222244
GTAGATC6900.048.18840838
GTATCAT6900.048.18840857