##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779948_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54245 Sequences flagged as poor quality 0 Sequence length 76 %GC 80 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.591317172089592 32.0 32.0 32.0 27.0 32.0 2 30.180588072633423 32.0 32.0 32.0 27.0 32.0 3 30.19128030233201 32.0 32.0 32.0 27.0 32.0 4 29.684616093649183 32.0 32.0 32.0 27.0 32.0 5 30.25104617937137 32.0 32.0 32.0 27.0 32.0 6 34.6276707530648 37.0 37.0 37.0 32.0 37.0 7 34.42781823209513 37.0 37.0 37.0 27.0 37.0 8 34.59922573509079 37.0 37.0 37.0 27.0 37.0 9 34.82199281039727 37.0 37.0 37.0 32.0 37.0 10-11 34.54522997511292 37.0 37.0 37.0 29.5 37.0 12-13 34.28120564107291 37.0 37.0 37.0 27.0 37.0 14-15 34.61360494054752 37.0 37.0 37.0 29.5 37.0 16-17 34.88702184533137 37.0 37.0 37.0 29.5 37.0 18-19 34.5871508894829 37.0 37.0 37.0 29.5 37.0 20-21 34.8952530187114 37.0 37.0 37.0 29.5 37.0 22-23 35.01210249792608 37.0 37.0 37.0 32.0 37.0 24-25 34.53942298829385 37.0 37.0 37.0 29.5 37.0 26-27 34.87237533413217 37.0 37.0 37.0 29.5 37.0 28-29 34.88091068301226 37.0 37.0 37.0 32.0 37.0 30-31 34.873868559314225 37.0 37.0 37.0 32.0 37.0 32-33 34.85606046640243 37.0 37.0 37.0 32.0 37.0 34-35 34.66032814084247 37.0 37.0 37.0 32.0 37.0 36-37 35.21139275509263 37.0 37.0 37.0 32.0 37.0 38-39 35.399575997787814 37.0 37.0 37.0 32.0 37.0 40-41 34.967066089040465 37.0 37.0 37.0 32.0 37.0 42-43 34.932316342520046 37.0 37.0 37.0 32.0 37.0 44-45 34.670513411374316 37.0 37.0 37.0 29.5 37.0 46-47 34.74688911420407 37.0 37.0 37.0 29.5 37.0 48-49 35.112010323532125 37.0 37.0 37.0 32.0 37.0 50-51 35.453820628629366 37.0 37.0 37.0 32.0 37.0 52-53 34.947460595446586 37.0 37.0 37.0 32.0 37.0 54-55 35.001308876394134 37.0 37.0 37.0 32.0 37.0 56-57 34.896193197529726 37.0 37.0 37.0 29.5 37.0 58-59 34.56855009678311 37.0 37.0 37.0 27.0 37.0 60-61 34.524582910867366 37.0 37.0 37.0 27.0 37.0 62-63 34.09950225827265 37.0 37.0 37.0 27.0 37.0 64-65 33.760051617660615 37.0 37.0 37.0 24.5 37.0 66-67 33.97514056595078 37.0 37.0 37.0 24.5 37.0 68-69 34.19792607613605 37.0 37.0 37.0 27.0 37.0 70-71 34.27502995667804 37.0 37.0 37.0 27.0 37.0 72-73 34.421568808185086 37.0 37.0 37.0 27.0 37.0 74-75 33.856733339478296 37.0 37.0 37.0 20.0 37.0 76 33.50561342059176 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.0477136119172066 11101 2 -1.4630018651898453 11101 3 -1.6306507693676053 11101 4 1.1591925278530795 11101 5 -0.26580920350355797 11101 6 0.3675658425564805 11101 7 -1.2099708943433853 11101 8 0.6044514632530991 11101 9 0.16265907385133715 11101 10-11 0.0011289395168034844 11101 12-13 0.5219495912833452 11101 14-15 0.3544372729786218 11101 16-17 1.0108190052349926 11101 18-19 -0.5667000651928191 11101 20-21 -1.07257172372843 11101 22-23 0.2454703307974313 11101 24-25 -0.1849177028242508 11101 26-27 -0.2617425252259551 11101 28-29 1.459197070040048 11101 30-31 -0.47984720383439594 11101 32-33 -0.5421084999622536 11101 34-35 -2.363784738199193 11101 36-37 -0.4042965495389552 11101 38-39 -1.1089733830813344 11101 40-41 1.0474767519533685 11101 42-43 -1.077507534084674 11101 44-45 -1.0963202984105536 11101 46-47 -1.2576675063157765 11101 48-49 -1.7583733332986142 11101 50-51 0.43935241407568526 11101 52-53 -1.7493372747441143 11101 54-55 -0.9062219585681461 11101 56-57 -0.1460270977762903 11101 58-59 -1.762215539814548 11101 60-61 -1.3848933588657388 11101 62-63 -1.2342547668689718 11101 64-65 0.909195861249259 11101 66-67 -0.4795534562473449 11101 68-69 0.4931844907888703 11101 70-71 0.8727223211925974 11101 72-73 0.031870261174240966 11101 74-75 -0.9110919711103023 11101 76 -0.20888156867091823 11102 1 0.904667340463746 11102 2 -0.7844304366184183 11102 3 0.262206373489537 11102 4 0.635383004043554 11102 5 -0.11104729874165287 11102 6 0.5818515568421958 11102 7 0.6233624389899504 11102 8 0.17588003468166846 11102 9 -0.34924568805342204 11102 10-11 0.21541465380251168 11102 12-13 0.682663876997637 11102 14-15 -0.5681817746404292 11102 16-17 -0.0010857566697666243 11102 18-19 0.3737761252833707 11102 20-21 -0.4594764856331892 11102 22-23 -0.3438153834882769 11102 24-25 0.3269870590805084 11102 26-27 -1.0474568109402398 11102 28-29 -1.1538981680551927 11102 30-31 -0.7834186324058265 11102 32-33 -0.9230608809146332 11102 34-35 -1.1733085477230105 11102 36-37 -1.219772740015145 11102 38-39 -0.9006400497480058 11102 40-41 -1.9763327718561499 11102 42-43 -1.2560789626561117 11102 44-45 -0.01893934602959746 11102 46-47 -0.358857982506251 11102 48-49 -1.1036114285367091 11102 50-51 -0.7630285383052637 11102 52-53 0.13756748716064493 11102 54-55 0.43306375571756917 11102 56-57 0.1456395688903811 11102 58-59 -0.5360250636240735 11102 60-61 -1.367036216008593 11102 62-63 0.43836428075007916 11102 64-65 -0.2098517577983614 11102 66-67 -0.9200296467235347 11102 68-69 0.11818449078887028 11102 70-71 -1.805849107378826 11102 72-73 -0.39074878644481004 11102 74-75 0.26747945746112833 11102 76 0.24349938371003077 11103 1 0.047524483320891875 11103 2 0.7512838490958664 11103 3 0.08363494491810641 11103 4 0.4568115754721269 11103 5 1.7818098441154895 11103 6 -0.1681484431578042 11103 7 -1.6266375610100496 11103 8 1.2115943203959532 11103 9 0.507897169089432 11103 10-11 0.3225575109453729 11103 12-13 0.7005210198547758 11103 14-15 1.3961039396452861 11103 16-17 1.3917713861873722 11103 18-19 0.8202046967119401 11103 20-21 1.022666371509672 11103 22-23 -0.1295296692025616 11103 24-25 1.505558487651939 11103 26-27 0.6846860462026143 11103 28-29 0.32824468908766846 11103 30-31 0.32372422473703466 11103 32-33 -1.1552037380574944 11103 34-35 -0.6554514048658646 11103 36-37 -0.5412013114437144 11103 38-39 0.09935995025199418 11103 40-41 0.09509579957241954 11103 42-43 -1.4167932483703964 11103 44-45 0.32034636825611074 11103 46-47 0.6054277317794572 11103 48-49 1.1463885714632909 11103 50-51 0.772685747409021 11103 52-53 1.7089960585892214 11103 54-55 1.2902066128604233 11103 56-57 -0.1936461453953413 11103 58-59 0.16040350780449586 11103 60-61 0.025820926848545867 11103 62-63 0.5990785664643639 11103 64-65 0.1472910993444998 11103 66-67 0.8299703532764653 11103 68-69 0.5824702050745856 11103 70-71 -1.0201348216645414 11103 72-73 1.0735369278408982 11103 74-75 -0.7325205425388717 11103 76 0.9577850979957461 11104 1 0.11245954825595561 11104 2 0.20799380580582394 11104 3 -0.3838975226143617 11104 4 0.8648202334807849 11104 5 -0.0060689437632994725 11104 6 0.5926740676647029 11104 7 1.0584273740548866 11104 8 1.2797761385777733 11104 9 1.1972911084833697 11104 10-11 0.28467872306659103 11104 12-13 0.8747634440971979 11104 14-15 0.3739177924591388 11104 16-17 -0.5497870553710626 11104 18-19 -0.09916759766034744 11104 20-21 0.620068968912264 11104 22-23 0.8450374303645418 11104 24-25 -0.07452809243464742 11104 26-27 0.39410163061820214 11104 28-29 0.22543083627381577 11104 30-31 0.16300993902274286 11104 32-33 1.098584140730381 11104 34-35 0.4543970799826198 11104 36-37 -0.03416667940908269 11104 38-39 -0.9076746817826375 11104 40-41 0.23145943593605978 11104 42-43 0.106475149898003 11104 44-45 0.40800870591844784 11104 46-47 0.3375705889223184 11104 48-49 -0.21941229433758025 11104 50-51 0.3051532798765564 11104 52-53 -0.13245415786099102 11104 54-55 -0.1941007464469351 11104 56-57 0.011981560232364075 11104 58-59 -0.3033410809400934 11104 60-61 0.40731443334205153 11104 62-63 -0.0740816066958132 11104 64-65 -0.04751409546069851 11104 66-67 0.2823513056574143 11104 68-69 0.11872561632999634 11104 70-71 0.4268348753051612 11104 72-73 0.5405282698322438 11104 74-75 0.5802500202316878 11104 76 -1.8322581920475436 11105 1 -0.8676540881076811 11105 2 -2.3781804366184183 11105 3 -1.3583293407961783 11105 4 -1.9940812816707307 11105 5 -1.3431901558845105 11105 6 -1.1592198717292348 11105 7 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11105 68-69 -1.1094940806396991 11105 70-71 -0.3951348216645414 11105 72-73 0.16505478498375936 11105 74-75 -0.42448482825315637 11105 76 -0.03775061628996923 11106 1 0.6231547354217319 11106 2 -1.4461951425007697 11106 3 -0.4667852231491203 11106 4 -1.2091548110824952 11106 5 -1.4828960382374525 11106 6 -1.4643669305527638 11106 7 -0.7463854601697122 11106 8 -0.5384056796040468 11106 9 0.6696618749717871 11106 10-11 -2.8392071949369786 11106 12-13 -1.1377142742628692 11106 14-15 -0.3297363964891673 11106 16-17 -0.48427903398069105 11106 18-19 -0.12412303438048866 11106 20-21 -2.3785941326920153 11106 22-23 -2.4415044591185335 11106 24-25 -1.5553658820959626 11106 26-27 -2.3037593319486405 11106 28-29 -0.9091502688955302 11106 30-31 -0.6899312374478441 11106 32-33 -1.6184390321751394 11106 34-35 -0.47162787545409657 11106 36-37 -0.962419798838674 11106 38-39 -0.5088333270589231 11106 40-41 -0.1990218474864065 11106 42-43 -1.1573394668577919 11106 44-45 -1.0010822031724587 11106 46-47 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0.9446066811232541 11107 28-29 1.8718954827384664 11107 30-31 0.5380099390227429 11107 32-33 0.872574039720277 11107 34-35 0.9834374840230211 11107 36-37 0.01633837109596925 11107 38-39 0.5695980454900962 11107 40-41 1.1506513551279767 11107 42-43 0.9145559579788127 11107 44-45 0.7211400190497628 11107 46-47 -0.3707627444110102 11107 48-49 0.654325079399797 11107 50-51 0.47308257280585053 11107 52-53 0.11375796335112653 11107 54-55 0.9965558192096324 11107 56-57 1.3996078228586342 11107 58-59 0.2635781109790969 11107 60-61 0.9186780697056918 11107 62-63 1.5256658680516608 11107 64-65 1.111576813630208 11107 66-67 1.3517957501018643 11107 68-69 0.8800892526936295 11107 70-71 0.36875406722434434 11107 72-73 0.7917908960948736 11107 74-75 1.6762096161912865 11107 76 1.854610494821145 11108 1 -0.280660844864439 11108 2 0.26286686067888 11108 3 -0.31018744890428707 11108 4 -0.7584393897788395 11108 5 -1.3262982639926193 11108 6 -1.5359090609184207 11108 7 0.34343965906716534 11108 8 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1.2596987344220096 11109 52-53 1.3220912966844551 11109 54-55 1.1392325868864006 11109 56-57 0.9665270147778173 11109 58-59 0.9542346766356644 11109 60-61 1.7103447363723632 11109 62-63 1.8956153630011556 11109 64-65 1.8009707530241457 11109 66-67 1.2671997905059058 11109 68-69 0.9823619799663632 11109 70-71 2.2753197237900054 11109 72-73 0.6920434213473996 11109 74-75 1.3681288081104839 11109 76 1.1071357473463976 11110 1 0.9588545325819737 11110 2 -0.17236147110117628 11110 3 -0.2020793407961783 11110 4 1.6600135459154757 11110 5 -0.21819015588451052 11110 6 1.9636249558569716 11110 7 0.9706530793840358 11110 8 1.254697768671818 11110 9 0.25789716908943205 11110 10-11 -0.030890764916691182 11110 12-13 0.9332796405444341 11110 14-15 1.0876064026994783 11110 16-17 0.17748567190166398 11110 18-19 0.6040716917858333 11110 20-21 1.493109721263366 11110 22-23 0.34399249828511813 11110 24-25 1.4045732659770636 11110 26-27 0.1668289033454755 11110 28-29 0.5462249846541738 11110 30-31 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6 0.4866134616041009 21612 7 -0.8052089895814802 21612 8 -0.5106279018262683 21612 9 0.09123050242276776 21612 10-11 -0.4135536001657414 21612 12-13 0.5060765754103329 21612 14-15 0.4080087015500453 21612 16-17 -0.25306988365389316 21612 18-19 -0.8782080017007559 21612 20-21 -0.18169870785541065 21612 22-23 -0.05016458983748606 21612 24-25 -0.6730129409194916 21612 26-27 0.5140511255676969 21612 28-29 -0.08643785059487641 21612 30-31 -0.9342122831994786 21612 32-33 -0.2663148491686087 21612 34-35 0.427881928467464 21612 36-37 0.47467170442929785 21612 38-39 0.7501536010456462 21612 40-41 -0.9049042004275805 21612 42-43 -0.5576662642434087 21612 44-45 -0.07052664761690153 21612 46-47 -0.3568738555221245 21612 48-49 0.09876952384423987 21612 50-51 -0.40191742719414947 21612 52-53 -0.1501309255377663 21612 54-55 0.7187780414318539 21612 56-57 -0.8503921771413658 21612 58-59 -0.4864218890209031 21612 60-61 -1.0535441525165297 21612 62-63 -0.6271119097261106 21612 64-65 -1.513423186369792 21612 66-67 -1.0787598054536929 21612 68-69 -1.0087996361952563 21612 70-71 0.5493096227799015 21612 72-73 -0.3470979927940192 21612 74-75 -1.032123717142042 21612 76 1.299054939265588 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 9.0 13 11.0 14 37.0 15 56.0 16 112.0 17 173.0 18 213.0 19 207.0 20 232.0 21 247.0 22 310.0 23 361.0 24 447.0 25 546.0 26 574.0 27 663.0 28 867.0 29 913.0 30 1190.0 31 1359.0 32 1931.0 33 2962.0 34 5160.0 35 8843.0 36 26820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.81961471103328 5.934187482717301 4.171813070329063 17.07438473592036 2 67.57120471932897 6.356346207023689 18.64687989676468 7.425569176882663 3 67.70762282237995 6.953636279841461 17.65508341782653 7.683657479952069 4 66.35632904391431 13.721839165222521 10.28538770694296 9.636444083920209 5 66.47801640704213 10.246105631855471 18.243156051248963 5.032721909853443 6 70.23892924302201 18.170421444636997 6.2202721138601085 5.370377198480882 7 68.97962945893632 9.840538298460688 6.015300949396258 15.164531293206748 8 69.30961378928934 18.06986819061665 5.94156143423357 6.678956585860448 9 68.04313761637017 6.363720158539958 4.995852152272099 20.59729007281777 10-11 69.66079823025164 11.790026730574246 11.175223522905336 7.37395151626878 12-13 68.95722146945774 7.0375798468047455 11.714551705702881 12.290646978034639 14-15 74.30407049627608 10.283164958336407 6.8920064891969615 8.520758056190546 16-17 74.15197994248211 7.8792124474596275 5.984071971093577 11.984735638964677 18-19 69.69001465586373 6.492824157287835 10.822295347915457 12.994865838932979 20-21 74.2787087734823 6.841435761296388 7.130874029828734 11.748981435392587 22-23 74.35729626591204 6.504005088167246 5.731562307004526 13.407136338916184 24-25 70.03493634947411 11.884811445111215 6.203736991049289 11.876515214365384 26-27 74.06646080103239 11.646771443056645 6.124348988339402 8.162418767571554 28-29 69.69378537322788 11.843002783769334 11.41437604852239 7.048835794480393 30-31 68.79908557944029 7.671177316470631 11.648722392242174 11.881014711846909 32-33 69.2212676523727 6.622174698573062 8.196600420338482 15.959957228715755 34-35 69.44731214512204 6.420986652901703 16.163999705036503 7.967701496939754 36-37 73.70031708576063 6.668940343632475 13.452179042843449 6.17856352776344 38-39 74.94331065759637 6.459819700237819 12.548162896594953 6.048706745570858 40-41 73.66228211675133 11.117461078285878 8.353996331357674 6.866260473605132 42-43 69.73682997649445 11.162833571461492 11.997050283449324 7.103286168594737 44-45 69.36435366123442 6.246773836737704 12.78113708428582 11.607735417742054 46-47 73.13091649844685 5.744361179474416 9.117053341813456 12.007668980265281 48-49 74.98893886881498 5.708465452400265 12.231767568763367 7.070828110021385 50-51 78.13747269258067 5.785946703291639 8.862813055942187 7.213767548185497 52-53 69.6903776489349 6.402610451021781 12.174730612884494 11.73228128715883 54-55 73.11223361109063 6.1085096969250054 8.25621266868225 12.523044023302116 56-57 72.86360829208492 6.892864714395008 7.4191853551973015 12.824341638322778 58-59 69.10505406177698 11.2077944822882 11.618903647441629 8.068247808493185 60-61 67.89908375274229 7.739247460501816 13.292037682281585 11.069631104474311 62-63 68.10556200800103 12.22738417860369 11.740685434065226 7.926368379330053 64-65 68.0579624645109 12.635964750562295 12.520740385678994 6.785332399247815 66-67 68.13233409843016 17.918014804162862 6.816736262824589 7.132914834582377 68-69 68.3455160526146 18.817001115341007 6.091974153585222 6.745508678459171 70-71 68.19681802260199 18.83561012480873 6.024740519514038 6.942831333075236 72-73 68.99450610228236 17.929832970760664 6.250691346189299 6.82496958076767 74-75 71.37642417314996 15.351572582131928 6.202758010397846 7.069245234320268 76 79.08485887579964 7.748465239754439 6.470881035340965 6.695794849104954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 3.5 14 3.5 15 6.5 16 15.5 17 19.0 18 17.0 19 17.0 20 19.5 21 20.0 22 31.0 23 46.5 24 73.0 25 95.0 26 102.5 27 133.5 28 149.0 29 141.0 30 152.0 31 197.0 32 244.0 33 257.0 34 270.5 35 300.0 36 318.5 37 321.0 38 357.5 39 448.0 40 511.0 41 804.5 42 1089.0 43 2443.5 44 3155.0 45 1662.0 46 812.0 47 758.5 48 624.5 49 511.5 50 479.0 51 495.0 52 492.0 53 538.0 54 603.0 55 559.0 56 542.5 57 572.5 58 575.0 59 556.0 60 544.0 61 409.0 62 267.0 63 276.0 64 293.0 65 282.0 66 241.5 67 220.0 68 195.5 69 158.5 70 118.0 71 90.0 72 88.0 73 80.5 74 71.5 75 68.0 76 65.5 77 46.5 78 35.5 79 41.0 80 40.0 81 35.0 82 34.5 83 38.0 84 33.5 85 30.0 86 37.0 87 43.0 88 40.5 89 43.0 90 55.0 91 68.0 92 74.0 93 88.5 94 111.0 95 144.5 96 170.0 97 228.5 98 453.0 99 16538.0 100 32457.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.005530463637201585 5 0.0 6 0.005530463637201585 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.217439395335977E-4 14-15 0.0018434878790671953 16-17 0.0018434878790671953 18-19 9.217439395335977E-4 20-21 0.0036869757581343907 22-23 0.0027652318186007926 24-25 0.006452207576735183 26-27 0.0046087196976679875 28-29 0.0036869757581343907 30-31 0.005530463637201585 32-33 0.005530463637201585 34-35 0.0018434878790671953 36-37 0.0018434878790671953 38-39 0.0036869757581343907 40-41 0.0027652318186007926 42-43 0.0046087196976679875 44-45 0.0018434878790671953 46-47 9.217439395335977E-4 48-49 0.0018434878790671953 50-51 0.0027652318186007926 52-53 0.0027652318186007926 54-55 0.0018434878790671953 56-57 9.217439395335977E-4 58-59 0.0027652318186007926 60-61 0.0036869757581343907 62-63 0.0036869757581343907 64-65 0.005530463637201585 66-67 0.006452207576735183 68-69 0.0027652318186007926 70-71 0.0036869757581343907 72-73 0.005530463637201585 74-75 0.005530463637201585 76 0.0036869757581343907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54245.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.521338372200205 #Duplication Level Percentage of deduplicated Percentage of total 1 69.14751171613479 11.424094386579409 2 10.365989734434278 3.4252004793068487 3 7.386744030350369 3.6611669278274492 4 5.4675295692925685 3.613236242971703 5 3.3586253068511494 2.7744492579961286 6 1.874581566614595 1.8582357820997326 7 0.9930819013613033 1.1484929486588624 8 0.36822137915643827 0.4866808000737395 9 0.23432269582682438 0.3484192091436999 >10 0.7141263110912742 2.1790026730574246 >50 0.02231644722160232 0.23780993639966816 >100 0.04463289444320464 2.586413494331275 >500 0.0 0.0 >1k 0.01115822361080116 7.294681537468891 >5k 0.0 0.0 >10k+ 0.01115822361080116 58.962116324085166 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 31984 58.962116324085166 No Hit CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG 3957 7.294681537468891 No Hit TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT 453 0.8351000092174394 No Hit ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC 440 0.8111346667895659 No Hit CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG 386 0.7115863213199373 No Hit TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG 124 0.2285924970043322 No Hit CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG 78 0.14379205456724123 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0073739515162687804 0.0 5 0.0 0.0 0.0 0.029495806065075122 0.0 6 0.0 0.0 0.0 0.029495806065075122 0.0 7 0.0 0.0 0.0 0.029495806065075122 0.0 8 0.0 0.0 0.0 0.03502626970227671 0.0 9 0.0 0.0 0.0 0.03502626970227671 0.0 10 0.0 0.0 0.0 0.03502626970227671 0.0 11 0.0 0.0 0.0 0.03502626970227671 0.0 12 0.0 0.0 0.0 0.04793068485574707 0.0 13 0.0 0.0 0.0 0.05714812425108305 0.0 14 0.0 0.0 0.0 0.08848741819522536 0.0 15 0.0 0.0 0.0 0.08848741819522536 0.0 16 0.0 0.0 0.0 0.08848741819522536 0.0 17 0.0 0.0 0.0 0.09217439395335976 0.0 18 0.0 0.0 0.0 0.09217439395335976 0.0 19 0.0 0.0 0.0 0.10323532122776292 0.0 20 0.0 0.0 0.0 0.10876578486496451 0.0 21 0.0 0.0 0.0 0.1106092727440317 0.0 22 0.0 0.0 0.0 0.1861922757857867 0.0 23 0.0 0.0 0.0 0.19540971518112268 0.0 24 0.0 0.0 0.0 0.24702737579500414 0.0 25 0.0 0.0 0.0 0.2617752788275417 0.0 26 0.0 0.0 0.0 0.2691492303438105 0.0 27 0.0 0.0 0.0 0.2912710848926168 0.0 28 0.0 0.0 0.0 0.34473223338556547 0.0 29 0.0 0.0 0.0 0.40741082127385014 0.0 30 0.0 0.0 0.0 0.45349801825053 0.0 31 0.0 0.0 0.0 0.47930684855747074 0.0 32 0.0 0.0 0.0 0.538298460687621 0.0 33 0.0 0.0 0.0 0.632316342520048 0.0 34 0.0 0.0 0.0 0.6913079546501981 0.0 35 0.0 0.0 0.0 0.792699787998894 0.0 36 0.0 0.0 0.0 1.0231357728822934 0.0 37 0.0 0.0 0.0 1.2922850032261037 0.0 38 0.0 0.0 0.0 1.5761821366024518 0.0 39 0.0 0.0 0.0 1.8711401972532031 0.0 40 0.0 0.0 0.0 2.2343073094294406 0.0 41 0.0 0.0 0.0 2.5735090791778044 0.0 42 0.0 0.0 0.0 3.0380680247027376 0.0 43 0.0 0.0 0.0 3.4712876762835285 0.0 44 0.0 0.0 0.0 3.9174117430177895 0.0 45 0.0 0.0 0.0 4.450179740068209 0.0 46 0.0 0.0 0.0 4.730389897686423 0.0 47 0.0 0.0 0.0 5.143331182597475 0.0 48 0.0 0.0 0.0 5.375610655359941 0.0 49 0.0 0.0 0.0 5.725873352382708 0.0 50 0.0 0.0 0.0 5.9876486312102495 0.0 51 0.0 0.0 0.0 6.260484837312195 0.0 52 0.0 0.0 0.0 6.50013826159093 0.0 53 0.0 0.0 0.0 6.780348419209144 0.0 54 0.0 0.0 0.0 7.0697760162226935 0.0 55 0.0 0.0 0.0 7.316803392017698 0.0 56 0.0 0.0 0.0 7.606230989031247 0.0 57 0.0 0.0 0.0 7.897502073923864 0.0 58 0.0 0.0 0.0 8.113190155774726 0.0 59 0.0 0.0 0.0 8.339939164899992 0.0 60 0.0 0.0 0.0 8.575905613420591 0.0 61 0.0 0.0 0.0 8.828463452852798 0.0 62 0.0 0.0 0.0 8.959351092266568 0.0 63 0.0 0.0 0.0 9.092082219559407 0.0 64 0.0 0.0 0.0 9.270900543828924 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 20 7.860762E-5 70.00001 1 CAATAAG 15 0.002213453 70.0 44 TGAACCA 15 0.002213453 70.0 47 AAAGGGG 30 8.2733095E-6 58.333332 70 TACTCCA 25 2.3706973E-4 55.999996 28 AAAAAGG 270 0.0 54.444447 70 ATGTGTA 20 0.006916529 52.500004 34 AAAATAT 20 0.006916529 52.500004 15 CATGTGT 20 0.006916529 52.500004 33 ATCCTTG 20 0.006916529 52.500004 30 CTATCCT 20 0.006916529 52.500004 34 TCTCGGT 670 0.0 48.58209 43 GATCTCG 685 0.0 48.540146 41 AGATCTC 685 0.0 48.540146 40 ATCTCGG 680 0.0 48.382355 42 CTTGTGT 660 0.0 48.257576 33 TCGGTGG 675 0.0 48.22222 45 CTCGGTG 675 0.0 48.22222 44 GTAGATC 690 0.0 48.188408 38 GTATCAT 690 0.0 48.188408 57 >>END_MODULE