FastQCFastQC Report
Wed 25 May 2016
SRR1779948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54245
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2215740.84616093649184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG667912.312655544289797No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG25074.621624112821459No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC17933.3053737671674805No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT7481.378928933542262No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT5370.9899529910590837TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC4150.765047469812886No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2430.44796755461332843No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1900.3502626970227671No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG1400.25808830306940733No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT1340.24702737579500417No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1340.24702737579500417No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC1250.23043598488339936No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT1190.21937505760899623No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT1160.21384459397179464No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC880.16222693335791316No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG780.14379205456724123No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG670.12351368789750208No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT630.11613973638123329No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT620.1142962485021661No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATATCGTATGCCGT590.10876578486496451No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATTG207.860762E-570.0000155
ATGGGTC150.00221345370.030
CACACGA150.00221345370.019
AAACACA252.8046143E-670.04
TCGAATG150.00221345370.040
CCATGGG150.00221345370.028
CTAGGGT150.00221345370.029
ACATCAC150.00221345370.08
GAGCACA150.00221345370.016
TCTGATT150.00221345370.054
CTTAAAC150.00221345370.01
TCACCGA150.00221345370.011
AAAATCC150.00221345370.029
TCGTAAG150.00221345370.040
TAGGGTC150.00221345370.030
AGTCTTA150.00221345370.069
ATTCCGT150.00221345370.044
GACCTAG150.00221345370.026
GCACACG150.00221345370.018
CTCGTAA150.00221345370.039
Produced by FastQC (version 0.11.5)