FastQCFastQC Report
Wed 25 May 2016
SRR1779947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574018
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410597.152911581169928No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG46960.8180928124205165No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC7810.13605845112871023No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG7760.1351873983045828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA150.002223896370.0034955
ACCGTCA200.006951300452.498047
GGTCTAA200.006951300452.498041
ACCCCGT200.006951300452.498048
GGTCCCA305.878012E-446.6649251
GGAATAT404.5929308E-543.7483671
GGTACAT503.4787263E-641.998431
TGGTCGC9600.040.10616349
GCCGTAT9450.040.0019954
GTACCAA350.001255474239.9985051
TGCGGGT350.001255474239.9985055
TCGCCGT9350.039.68457452
GTCGCCG9850.039.09164451
GATCTCG9850.039.08483541
ATCTCGG9900.038.88743642
AGATCTC9900.038.88743640
TCGGTGG9900.038.88743645
TAGATCT9950.038.69202439
CTCGGTG9950.038.3402844
CGGTGGT10250.037.90102446
Produced by FastQC (version 0.11.5)