FastQCFastQC Report
Wed 25 May 2016
SRR1779947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574018
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT267114.6533383970537505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86081.499604542017846No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC33300.5801211808688926No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG30450.530471169893627No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT11950.20818162496646447No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT10350.18030793459438554RNA PCR Primer, Index 19 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATACG150.002222886570.011525
AATCACG207.92905E-569.9932136
GTCCGCA150.00222519269.993212
AGGGGGG38400.058.2365370
GTCGAGC200.006952940552.4949078
CGTCTTA200.006952940552.49490748
GCCGTCT44250.051.17017746
CCGTCTT44350.050.97588347
TATGCCG45050.050.8052843
CGTCTTC44950.050.6068848
CGTATGC45850.050.5294441
ATGCCGT45500.050.22589544
TGCCGTC45600.050.19249345
TCGTATG46600.050.091740
ATCGTAT352.0873566E-549.99514839
GTATGCC45950.049.96250542
CTCGTAT46450.049.9520939
CTTGAAA46100.049.72835557
TCTTCTG46150.049.5943250
GTCTTCT46150.049.44265449
Produced by FastQC (version 0.11.5)