FastQCFastQC Report
Wed 25 May 2016
SRR1779946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168331
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3657921.730400223369433No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG74754.440655613048102No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC13160.7817930149526826No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG8780.5215913883954827No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT8530.5067396973819439No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG2290.13604148968401542No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2110.12534827215426747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGC150.00222391769.98157531
ATGCGCC200.006940846352.5017820
TGCCACG200.0069490152.48618730
GCAATCT200.0069490152.48618729
GTCGCCG13200.043.22116551
GCCGTAT13050.043.1815454
TCGCCGT13200.042.95600552
TGGTCGC13450.042.417849
CGCCGTA13300.042.38245853
GTATCAT13100.042.21517657
GGTCGCC13550.042.1047550
CGGTGGT13650.042.0527146
CGTATCA13250.042.00142756
TCGGTGG13600.041.94995545
TAGATCT13450.041.89733539
CATTAAA13300.041.8435361
AGATCTC13500.041.75456640
GGTGGTC13750.041.74687247
GTAGATC13600.041.70498738
CTCGGTG13600.041.69259344