FastQCFastQC Report
Wed 25 May 2016
SRR1779946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168331
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT138338.21773767161129No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131647.820306420088992No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT82364.892741087500223No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG27261.619428388116271No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC22001.3069488091914145No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC8280.4918880063684051No Hit
CATAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT7280.43248124231424995No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT5750.3415888933113924Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC5190.3083211054410655No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4610.2738651822896555No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT4110.2441618002625779No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC3530.20970587711116787No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT2350.13960589552726474No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT1930.11465505462451954No Hit
AAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT1750.10396183709477161Illumina PCR Primer Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG150.002222905269.989617
GCTTAAT150.002222905269.989648
CATATAC9350.062.8596761
ATATACA9600.061.951552
AGGGGGG29400.060.96144570
CGTCTTA252.3869074E-455.99167648
TTATAGC252.3869074E-455.99167631
AACTCGT252.3869074E-455.99167637
CAACTCG252.3869074E-455.99167636
ACTTATA1600.054.6631282
CGTCTAC452.6882844E-854.4363548
GAATCTC653.6379788E-1253.83815435
ACGAACG200.006929542452.52341529
AGTCTGA200.00693769852.507870
GTCCATA200.00693769852.507830
TATCGCT200.00693769852.507819
CACCCGT200.00693769852.507823
GGGTAGT200.006945860652.492242
CGTTCTC200.006945860652.492241
GTAGCAA200.006945860652.492234