FastQCFastQC Report
Wed 25 May 2016
SRR1779945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315768
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295169.347368954422235No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG56551.7908717792809912No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC8630.2733019178637481No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG7230.2289655696587368No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6160.1950799321020496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT150.002224842869.9885948
TAGCGTA150.002224842869.9885947
ATAGCGT150.002224842869.9885946
CCCGTAC200.006947515552.4997613
GTTCTAT200.006951868552.4914471
TGGTCGC9850.046.90334349
GCCGTAT9650.046.78735454
CGTATCA9700.046.5535556
TCGCCGT9800.046.0712252
GGTGGTC10150.045.86186247
TCGGTGG9950.045.72843645
CTCGGTG10050.045.27342644
GTAGATC10100.045.04930538
ATCTCGG9950.045.0249242
GATCTCG9950.045.0249241
GTGGTCG10350.044.9756448
GTATCAT10150.044.83448457
CGCCGTA10100.044.7027753
CCGTATC10150.044.48960555
GTCGCCG10300.044.17455751
Produced by FastQC (version 0.11.5)