FastQCFastQC Report
Wed 25 May 2016
SRR1779945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315768
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT117603.724253249220947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98743.1269793012591522No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT62511.9796179473537532No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG20270.6419269843682703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC16770.5310861138557421No Hit
CACTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC5720.18114565123761747No Hit
CATAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT5400.1710116287907578No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT4160.13174229180917635RNA PCR Primer, Index 34 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC4070.12889209799599707No Hit
CAAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT3580.1133743761242431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27050.058.6024170
CGTATGA509.804353E-1055.98923541
TGACGTC509.804353E-1055.98923545
CATATAC9300.054.183131
AAGGGGG30450.051.8291169
ATCTCGT31000.051.24820737
TCTCGTA31500.051.10128438
TCGTATG31850.051.08907340
CTCGTAT31250.051.06217639
CGTATGC31550.051.020341
CGTCTTC30250.051.0149848
CCGTCTT30250.050.899347
GATCTCG31600.050.71809436
TATGCCG31350.050.6760643
GCCGTCT30600.050.66019446
GGATCTC31650.050.6379735
GGGATCT31550.050.46573334
CTTGAAA31850.050.4458457
TGCCGTC31500.050.43474645
GTCTTCT30400.050.41793449