FastQCFastQC Report
Wed 25 May 2016
SRR1779944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228960
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2904912.687368972746333No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG61272.6760132774283716No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC8740.38172606568832984No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG7720.33717679944095036No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6230.272099930118798No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCC200.006944964652.58
TGCGTTG200.006944964652.512
CTTTATA352.0821912E-550.0000042
GCCGTAT11000.046.45454854
CTCGGTG11250.046.35555344
TGGTCGC11250.046.0444449
TCGCCGT11100.046.03603452
TCTCGGT11350.045.9471443
TCGGTGG11400.045.74561345
CGGTGGT11450.045.54585346
GATCTCG11300.045.53097541
AGATCTC11500.045.0434840
GTATCAT11350.045.0220357
GTCGCCG11400.044.8245651
CGTATCA11400.044.8245656
CCGTATC11400.044.8245655
TAGATCT11450.044.62882239
GTGGTCG11700.044.5726548
ATCTCGG11600.044.35344742
CGCCGTA11450.044.32314353