FastQCFastQC Report
Wed 25 May 2016
SRR1779944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228960
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT107154.679856743535988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104844.578965758211042No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT64432.8140286512928023No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG21370.9333508036338225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC16540.722396925227114No Hit
CACTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC6560.286512928022362No Hit
CATAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT5840.2550663871418588No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC3800.16596785464709993No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT3630.15854297693920336TruSeq Adapter, Index 10 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3230.14107267645003496No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3220.14063591893780572No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC2460.10744234800838573No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT2320.10132774283717678No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG24500.062.13936670
AACTCGT401.0626536E-861.24656337
CATATAC8850.060.5182951
ATATACA9350.058.4051972
TCCGGAT252.3876439E-455.9968574
TGCCGTA252.3876439E-455.99685745
AAGGGGG28000.053.74698369
GCCGTCT27400.052.8802446
CCGTCTT27200.052.75438747
ATGCCGT28200.052.74526644
TATGCCG28800.052.74009343
TCTTCTG27300.052.6893550
CTTGAAA28800.052.61857257
TGCCGTC28050.052.5282445
TGTTCGT200.00693451252.51999328
TTGTTCG200.00693451252.51999327
TCGTTTA200.00694050752.5085232
AATCGAT200.00694050752.5085222
CGTCTAC200.00694650652.4970548
TAAGTGC200.00694650652.4970515
Produced by FastQC (version 0.11.5)