FastQCFastQC Report
Wed 25 May 2016
SRR1779943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46373
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2480853.49664675565523No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG575912.418864425419965No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6151.326202747288293No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC5661.2205378129515019No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG5571.2011299678692344No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG1760.37953119271990166No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG1230.26524054945765857No Hit
TATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGT680.14663705173268926No Hit
CTTTACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG650.14016777003860006No Hit
TCTTAATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG560.12075992495633235No Hit
ACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGC520.11213421603088003No Hit
CTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT510.10997778879951695No Hit
CTTAATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT490.1056649343367908No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA207.8487436E-570.02
AAATAGG150.002211421570.070
CTTAATA150.002211421570.02
TTAATAC150.002211421570.03
TCTTTAT207.8487436E-570.01
TTTATAC207.8487436E-570.03
TCTTAAT150.002211421570.01
AAAAAGG5450.056.51376370
AAGGGGG452.6286216E-854.44444770
CATCCGC200.00691022952.510
GGGGGCT200.00691022952.513
TCCGCCT200.00691022952.512
AAAATAG200.00691022952.569
TGGTCGC9100.049.2307749
GTGGTCG9150.048.9617548
CATTAAA9100.048.84615761
GTATCAT9050.048.72928257
CGCCGTA9050.048.72928253
GGTGGTC9200.048.69565647
CCGTATC9000.048.61110755
Produced by FastQC (version 0.11.5)