FastQCFastQC Report
Wed 25 May 2016
SRR1779943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46373
Sequences flagged as poor quality0
Sequence length76
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1022322.045155586224745No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG916019.752873439285793No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT546611.787031246630582No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG18443.9764518146335153No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC11122.3979470812757424No Hit
CATAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4110.8862915920902249No Hit
CACTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3620.7806266577534341No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3370.7267159769693572No Hit
CAAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2900.6253638970952925No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2830.6102689064757509No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT2010.43344187350397867RNA PCR Primer, Index 14 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1910.41187760119034783No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1560.3364026480926401No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC910.19623487805404005No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT890.1919220235913139No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC820.17682703297177238No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG810.1746706057404093No Hit
CAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC800.1725141785090462No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC690.14879347896405234No Hit
CAAAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT670.1444806245013262No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG620.1336984883445108No Hit
TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG600.12938563388178465No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT560.12075992495633235No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT560.12075992495633235No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG530.11429064326224311No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT510.10997778879951695No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT500.10782136156815389No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT500.10782136156815389No Hit
AAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT500.10782136156815389RNA PCR Primer, Index 14 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT470.10135207987406465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAACG301.0027543E-770.024
TGCCGAC252.7981841E-670.045
TCGAATG207.8487436E-570.040
CTCTCGT150.002211421570.037
AAGAGGG150.002211421570.069
GAACGGT207.8487436E-570.026
TCTATAC150.002211421570.01
ACTTCTG150.002211421570.050
ATGTGTA150.002211421570.08
AACTCGT150.002211421570.037
CAATACA150.002211421570.01
AAAAGAG150.002211421570.067
CGAATGC207.8487436E-570.041
ATGCCGA252.7981841E-670.044
CAGATTG150.002211421570.054
AGACCAC150.002211421570.017
ACGGTTC150.002211421570.028
TGTATAA207.8487436E-570.011
GCCGACT150.002211421570.046
GACCACG150.002211421570.018
Produced by FastQC (version 0.11.5)