##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779943_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 46373 Sequences flagged as poor quality 0 Sequence length 76 %GC 60 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.09022491536023 32.0 32.0 32.0 27.0 32.0 2 21.169279537662003 22.0 13.0 32.0 8.0 32.0 3 29.338041532788477 32.0 27.0 32.0 27.0 32.0 4 30.963362301339142 32.0 32.0 32.0 32.0 32.0 5 28.315636253854613 32.0 27.0 32.0 13.0 32.0 6 33.87272766480495 37.0 32.0 37.0 27.0 37.0 7 34.966057835378344 37.0 37.0 37.0 32.0 37.0 8 34.8552390399586 37.0 37.0 37.0 32.0 37.0 9 35.10674314795247 37.0 37.0 37.0 32.0 37.0 10-11 35.194445043452006 37.0 37.0 37.0 29.5 37.0 12-13 35.32314062061975 37.0 37.0 37.0 32.0 37.0 14-15 35.47902874517499 37.0 37.0 37.0 32.0 37.0 16-17 33.93924266275634 37.0 34.5 37.0 24.5 37.0 18-19 34.66509606883316 37.0 37.0 37.0 27.0 37.0 20-21 35.405429883768576 37.0 37.0 37.0 32.0 37.0 22-23 34.83292001811399 37.0 37.0 37.0 29.5 37.0 24-25 34.888124555236885 37.0 37.0 37.0 32.0 37.0 26-27 34.750760140599056 37.0 37.0 37.0 29.5 37.0 28-29 34.82245056390572 37.0 37.0 37.0 29.5 37.0 30-31 34.922217669764734 37.0 37.0 37.0 32.0 37.0 32-33 35.0560778901516 37.0 37.0 37.0 32.0 37.0 34-35 34.065124102387166 37.0 37.0 37.0 24.5 37.0 36-37 32.671500657710304 37.0 32.0 37.0 20.0 37.0 38-39 32.31832100575766 37.0 29.5 37.0 13.0 37.0 40-41 33.32016475104048 37.0 34.5 37.0 20.0 37.0 42-43 34.2459297436008 37.0 37.0 37.0 27.0 37.0 44-45 34.4853686412352 37.0 37.0 37.0 27.0 37.0 46-47 34.05460073749811 37.0 34.5 37.0 27.0 37.0 48-49 33.12500808660212 37.0 34.5 37.0 17.5 37.0 50-51 33.501326202747286 37.0 37.0 37.0 22.0 37.0 52-53 32.547322795592265 37.0 32.0 37.0 17.5 37.0 54-55 32.63715955405085 37.0 32.0 37.0 17.5 37.0 56-57 32.95060703426563 37.0 32.0 37.0 17.5 37.0 58-59 32.699372479675674 37.0 32.0 37.0 17.5 37.0 60-61 33.34287192978673 37.0 34.5 37.0 22.0 37.0 62-63 33.99461971405775 37.0 37.0 37.0 27.0 37.0 64-65 34.15704181312402 37.0 37.0 37.0 27.0 37.0 66-67 34.027440536519094 37.0 37.0 37.0 27.0 37.0 68-69 33.33907661785953 37.0 32.0 37.0 22.0 37.0 70-71 30.68505380285942 37.0 27.0 37.0 13.0 37.0 72-73 33.11183231621849 37.0 34.5 37.0 17.5 37.0 74-75 34.33431091367822 37.0 37.0 37.0 27.0 37.0 76 34.4129126862614 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.31635031174625183 11101 2 -1.914026672053275 11101 3 -0.7583370868186314 11101 4 -0.8578985206273835 11101 5 -2.793583280566441 11101 6 -1.7246915471987734 11101 7 -1.89760870313647 11101 8 0.1266919162476725 11101 9 -2.4696964650500703 11101 10-11 -2.0453968914741125 11101 12-13 0.9975400427467704 11101 14-15 -1.940735757027788 11101 16-17 -0.48470594828907565 11101 18-19 -3.612404351609811 11101 20-21 -1.2011102351348626 11101 22-23 -0.8989376241929108 11101 24-25 -3.3944954655837307 11101 26-27 -0.6700588515545576 11101 28-29 -2.70051866387783 11101 30-31 -2.3988902813857678 11101 32-33 -0.6024941842910465 11101 34-35 -1.7662293255072825 11101 36-37 -2.588544196383623 11101 38-39 -1.3813748846725176 11101 40-41 -1.8017813061435959 11101 42-43 -3.547736011110416 11101 44-45 -0.774691301345527 11101 46-47 -2.569820487981616 11101 48-49 -1.6747572222931275 11101 50-51 -0.010278968357944507 11101 52-53 -1.256740139165835 11101 54-55 -0.5054987390592842 11101 56-57 0.047013774910674044 11101 58-59 -0.8898009036872523 11101 60-61 -2.428842527670234 11101 62-63 -3.268194953768919 11101 64-65 0.6501661212775076 11101 66-67 0.1334310236364118 11101 68-69 -0.27852134209686596 11101 70-71 -2.4124409261877418 11101 72-73 -2.2329638661072195 11101 74-75 -0.8175117075193654 11101 76 -0.3269394097903273 11102 1 1.8334310570878678 11102 2 -0.9326602124259473 11102 3 -0.6527470247068301 11102 4 -0.5162836137950855 11102 5 -1.1289870072745174 11102 6 -0.03369775837889932 11102 7 -0.28270187083833775 11102 8 -0.7382149160504596 11102 9 -0.46348528492585217 11102 10-11 -0.16573850638094711 11102 12-13 -2.2610003299240375 11102 14-15 -1.2481891731768542 11102 16-17 -1.698991662574791 11102 18-19 -1.9617832335973873 11102 20-21 -0.23993011091125993 11102 22-23 -1.2009562577332815 11102 24-25 -1.8230668941551613 11102 26-27 -0.9736302801259882 11102 28-29 -1.6026925769213065 11102 30-31 0.2144637558813116 11102 32-33 -0.8664693395705498 11102 34-35 -0.2553597602898918 11102 36-37 -1.4697553765078482 11102 38-39 -1.5739214685234515 11102 40-41 0.5592435385769008 11102 42-43 -1.3225807316073102 11102 44-45 -1.3135111771219243 11102 46-47 -1.4269633451244772 11102 48-49 -0.7128007005539985 11102 50-51 -1.7959932540722292 11102 52-53 0.8022660720142909 11102 54-55 -1.5823620930965525 11102 56-57 -2.147085603971316 11102 58-59 -1.3525338229419113 11102 60-61 -1.121389111521168 11102 62-63 -0.5787539599800979 11102 64-65 -2.0571320153684525 11102 66-67 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11103 46-47 -0.35315382131494744 11103 48-49 -0.8711340338873299 11103 50-51 -1.226945635024613 11103 52-53 -0.5096386898904726 11103 54-55 0.3473998116653547 11103 56-57 0.5767239198382086 11103 58-59 0.21889474848666168 11103 60-61 0.6595632694312101 11103 62-63 0.6426746114484772 11103 64-65 1.0892965560601198 11103 66-67 1.2247353714625007 11103 68-69 -1.1705503276041114 11103 70-71 -0.13345541894135948 11103 72-73 1.3221085976608933 11103 74-75 -1.2443233017222681 11103 76 -2.3269394097903273 11104 1 -0.2001187264619162 11104 2 -1.9759502557159898 11104 3 -1.2155175874773931 11104 4 0.17635707884560858 11104 5 -0.9385108167983276 11104 6 -1.2793687540498908 11104 7 -0.24806983620629808 11104 8 1.1741227462872033 11104 9 0.3287225072819453 11104 10-11 -0.9768856925281355 11104 12-13 0.6042594103357004 11104 14-15 -1.0079294329171162 11104 16-17 -0.24228170586483344 11104 18-19 -0.8113503331644978 11104 20-21 -0.8124409334220815 11104 22-23 0.40672772495070575 11104 24-25 0.014595443507175787 11104 26-27 -1.367028548524253 11104 28-29 -1.2314804557091819 11104 30-31 0.24801353943109206 11104 32-33 -0.2928762659774833 11104 34-35 -2.0962688511989853 11104 36-37 0.38576410401162775 11104 38-39 -0.9202418148437985 11104 40-41 0.52298812732149 11104 42-43 0.6828305238039434 11104 44-45 -0.17390078751154192 11104 46-47 -0.5395174576785848 11104 48-49 -1.4665885793418774 11104 50-51 0.08063012255114899 11104 52-53 -2.2778205080722884 11104 54-55 -0.15108503681949514 11104 56-57 -0.5096397165254274 11104 58-59 -1.4780749484830338 11104 60-61 0.24441175427970308 11104 62-63 0.18964430841817403 11104 64-65 1.0862662530298124 11104 66-67 0.6307959775231069 11104 68-69 0.18854058148679798 11104 70-71 -0.9440614795474218 11104 72-73 -0.19607322052092258 11104 74-75 -2.7094748168737794 11104 76 0.46093937808846164 11105 1 0.5453358189926298 11105 2 1.6482921685264351 11105 3 0.6663005943407896 11105 4 0.22181162430015533 11105 5 -0.7718441501316597 11105 6 -0.15512632980746588 11105 7 -1.7874637756002372 11105 8 0.2832136553781126 11105 9 -0.4682471896877516 11105 10-11 0.3640233983809509 11105 12-13 0.9163806224569129 11105 14-15 -0.5958082207959023 11105 16-17 1.6819607183775958 11105 18-19 -0.6022594240735799 11105 20-21 0.7481651271839809 11105 22-23 0.4930913613143346 11105 24-25 0.372171201082935 11105 26-27 -1.0700588515545633 11105 28-29 -0.05269257692130935 11105 30-31 -0.4974410060234504 11105 32-33 -0.1307550538562623 11105 34-35 -0.08869309362322753 11105 36-37 0.743339861587387 11105 38-39 -0.3929690875710712 11105 40-41 0.7032911576245198 11105 42-43 0.7631335541069717 11105 44-45 -0.8587492723600221 11105 46-47 0.08017951201838258 11105 48-49 0.42886596611266725 11105 50-51 -0.010278968357944507 11105 52-53 0.2070279767761889 11105 54-55 0.29739981166535756 11105 56-57 0.7267239198382072 11105 58-59 0.7355614151533274 11105 60-61 0.15956326943121013 11105 62-63 -0.6906587218848657 11105 64-65 1.072629889393454 11105 66-67 0.19140203812916923 11105 68-69 -1.2372169942707814 11105 70-71 -0.5167887522746923 11105 72-73 0.7054419309942332 11105 74-75 0.6223433649444061 11105 76 0.07306059020967126 11106 1 0.8355318974240014 11106 2 3.3404490312715325 11106 3 1.8976731433603966 11106 4 -0.65073739530769 11106 5 -0.6836088560140112 11106 6 -1.4433616239251137 11106 7 0.41057544008603486 11106 8 -0.1226686975630642 11106 9 0.6709684965867524 11106 10-11 -0.8075452290700298 11106 12-13 -0.2914625147979848 11106 14-15 -0.1095337109919825 11106 16-17 -0.38176477181848867 11106 18-19 -0.30422020838730646 11106 20-21 0.4785572840467225 11106 22-23 0.6068168515104162 11106 24-25 0.7231515932397983 11106 26-27 -0.06711767508396349 11106 28-29 0.5002485995492876 11106 30-31 -0.699401790337177 11106 32-33 0.28689200496726386 11106 34-35 0.009346122063050188 11106 36-37 0.3002026066854313 11106 38-39 0.19918777517403186 11106 40-41 -1.7947480580617565 11106 42-43 0.2719570835187426 11106 44-45 0.5520350413654711 11106 46-47 0.415473629665442 11106 48-49 -0.7201536417304695 11106 50-51 0.019132796347939518 11106 52-53 -0.33218770949831367 11106 54-55 -0.4594629334326896 11106 56-57 0.7835866649362515 11106 58-59 0.04242416025136464 11106 60-61 -2.6865151619413368 11106 62-63 0.4044393173308265 11106 64-65 1.374590673707175 11106 66-67 0.7894412538154398 11106 68-69 0.21572418219981415 11106 70-71 1.1655641889017758 11106 72-73 1.0387752643275618 11106 74-75 0.12430414925812983 11106 76 0.6730605902096727 11107 1 -0.5052388936510503 11107 2 1.1264530880666648 11107 3 0.1157258816971094 11107 4 0.22755875073693588 11107 5 0.7281558498683403 11107 6 1.5931495322614992 11107 7 1.1458695577330928 11107 8 -0.12368289634602547 11107 9 0.24094821261109445 11107 10-11 0.5048279960821063 11107 12-13 0.8738518868247311 11107 14-15 0.2616630435719145 11107 16-17 -0.019188706909758935 11107 18-19 -0.3447881597057645 11107 20-21 0.9780501846552454 11107 22-23 0.31320630384307435 11107 24-25 0.1572286723473013 11107 26-27 0.5368377001695848 11107 28-29 1.2162729403200743 11107 30-31 -0.41238353475908696 11107 32-33 0.74683115304029 11107 34-35 0.28774368798596583 11107 36-37 -0.3164302533551364 11107 38-39 0.8127780388657087 11107 40-41 -1.0731456239846722 11107 42-43 1.5062370023828393 11107 44-45 -0.009323985003703683 11107 46-47 1.1715588223632096 11107 48-49 -0.1395248384850305 11107 50-51 0.19661758336619783 11107 52-53 -0.5228570806950756 11107 54-55 -1.6876576595990151 11107 56-57 -0.14339102269052262 11107 58-59 2.3395844036590745 11107 60-61 0.7825517751783408 11107 62-63 -0.5418081471722118 11107 64-65 1.0272275905428785 11107 66-67 -0.6821611802616445 11107 68-69 0.3196795574533624 11107 70-71 2.1452802132425433 11107 72-73 1.8577407815689426 11107 74-75 0.7769410660938334 11107 76 0.8109916246924342 11108 1 -0.13505633787011817 11108 2 -0.688962733434348 11108 3 0.4859084374780416 11108 4 -1.6801491600135705 11108 5 -3.7718441501316597 11108 6 -3.3845380945133527 11108 7 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0.5767675705064832 21611 62-63 -0.37829313048701607 21611 64-65 0.5183288141246294 21611 66-67 0.25054182307540174 21611 68-69 1.6289120379872841 21611 70-71 1.4767596348220806 21611 72-73 0.8532913933598181 21611 74-75 -0.586796420001825 21611 76 1.253705751499993 21612 1 0.8829702275947788 21612 2 2.9751738889565438 21612 3 -0.1734843518957696 21612 4 0.11966108666574726 21612 5 1.8571881079328563 21612 6 -1.5970618136784367 21612 7 -1.6605820551701314 21612 8 -1.2716250542993066 21612 9 -0.5510428886124856 21612 10-11 -1.3806002575330254 21612 12-13 -1.5115763667903934 21612 14-15 0.8923638222148469 21612 16-17 -1.8395446579664991 21612 18-19 -0.8108615746112164 21612 20-21 -0.22495315238592184 21612 22-23 -1.1773387462125484 21612 24-25 -0.6686890139708268 21612 26-27 -1.4281233676835896 21612 28-29 0.9376300037238536 21612 30-31 -0.6576560597868948 21612 32-33 -1.6130131183723933 21612 34-35 0.930124110677852 21612 36-37 -1.9910687405631506 21612 38-39 0.6403642457622638 21612 40-41 -1.6671389499023626 21612 42-43 -0.6900922523446411 21612 44-45 -1.6533729282739955 21612 46-47 -0.3762721008848402 21612 48-49 -0.5071555392636782 21612 50-51 -0.6715692909385851 21612 52-53 1.2124043208622197 21612 54-55 -0.13593352166797956 21612 56-57 -2.7012330694091062 21612 58-59 0.3963141033253663 21612 60-61 -0.4232324294935168 21612 62-63 -1.4911963562934645 21612 64-65 -2.3042518310366553 21612 66-67 -1.2817162414407264 21612 68-69 -0.7904428007223956 21612 70-71 1.057404796112401 21612 72-73 -2.0499344130917905 21612 74-75 -0.19969964580828758 21612 76 -1.3914555388225835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 2.0 17 3.0 18 2.0 19 13.0 20 35.0 21 57.0 22 116.0 23 216.0 24 337.0 25 384.0 26 601.0 27 807.0 28 997.0 29 1322.0 30 1765.0 31 2523.0 32 4002.0 33 6402.0 34 9832.0 35 12648.0 36 4308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.108037004291287 6.52319237487331 9.20578785068898 54.162982770146414 2 25.095967220185468 26.340306232477893 35.12184602113436 13.441880526202285 3 25.50677132752523 10.588286034676099 52.76459932718019 11.140343310618476 4 24.024755784616048 47.37239341858409 19.500571453216313 9.10227934358355 5 24.19295710866237 19.312962284087725 50.65231923748733 5.841761369762578 6 27.93927369964634 56.393944621754514 9.73000948848443 5.936772190114724 7 27.1170724343907 14.935415004420676 9.919565264270156 48.02794729691847 8 26.12727233519505 56.44663920815992 5.39538093287042 12.03070752377461 9 25.883596058051022 11.991891833610074 4.972721195523257 57.15179091281565 10-11 26.662066288573094 32.76259892609924 27.800659866732797 12.774674918594872 12-13 26.8076251266901 8.192267051948331 32.97608522200418 32.02402259935738 14-15 27.510620404114462 6.142582968537727 10.351928924158454 55.99486770318936 16-17 51.52135941172665 27.959157268237984 5.061134712009143 15.458348608026224 18-19 53.216311215578024 11.914260453281004 5.12151467448731 29.747913656653658 20-21 29.257326461518556 6.991137084079098 5.2422745994436415 58.50926185495871 22-23 27.56267184214782 30.92026524340935 5.15715132891261 36.35991158553022 24-25 51.66598373913606 31.07787530462162 5.049709935517264 12.206431020725054 26-27 53.640177290815174 33.300262053942134 5.474015680100076 7.5855449751426205 28-29 30.302703518780127 11.636885184026918 5.8125114578727715 52.24789983932019 30-31 72.57483609385783 5.703286749482402 6.98865596963423 14.733221187025535 32-33 34.45044695327748 5.998555084700072 51.65464367741727 7.896354284605183 34-35 25.790103621914795 7.199620448345392 14.290335450340194 52.71994047939962 36-37 25.831841804498307 27.760765962952583 7.119444504345202 39.28794772820391 38-39 26.269596532465012 11.2252819528605 30.833674767644965 31.67144674702952 40-41 26.292239018394326 6.045543743126388 33.723287257671494 33.9389299808078 42-43 50.2803355400772 6.012119121040261 31.587346085006363 12.120199253876178 44-45 28.621180428266445 30.028249196730854 34.06292454661117 7.287645828391522 46-47 49.98382679576478 8.561016108511419 12.856619153386669 28.59853794233714 48-49 49.373557889289025 5.846074224225303 7.890367239557501 36.890000646928165 50-51 30.269769046643518 6.325879283203588 31.835335216613114 31.569016453539778 52-53 24.826946714683114 7.026718133396588 55.2562913764475 12.890043775472796 54-55 25.263101142980375 6.862195384947165 36.51714470562864 31.357558766443823 56-57 49.37679253013607 5.938800595173916 13.219977141871347 31.464429732818665 58-59 28.306789877184848 7.987837094704607 51.355926720652135 12.349446307458406 60-61 49.08675306751773 29.90425463092748 13.822698553037327 7.186293748517457 62-63 27.33810622560542 59.70607896836522 5.45144804088586 7.50436676514351 64-65 24.64363503051476 62.7126868085657 5.072135601992625 7.571542558926915 66-67 25.054990080220822 62.73721211075649 4.921073061330113 7.286724747692573 68-69 26.323507213249087 61.81075194617558 4.598581070881763 7.267159769693572 70-71 47.72065641644922 39.89282556660125 4.688072800983331 7.698445215966188 72-73 79.84926573652771 7.765294460138443 4.872447329264874 7.512992474068962 74-75 82.53607091250242 4.925917139344793 5.251579787348761 7.2864321608040195 76 82.6774200504604 4.810989153171026 5.043883294158238 7.467707502210338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 2.5 15 3.5 16 3.5 17 4.0 18 3.0 19 5.0 20 12.5 21 17.0 22 14.0 23 22.5 24 38.0 25 42.0 26 48.5 27 59.5 28 68.0 29 72.0 30 88.0 31 113.5 32 127.0 33 131.0 34 154.5 35 168.5 36 208.5 37 258.0 38 249.0 39 276.5 40 334.0 41 379.0 42 403.0 43 397.0 44 437.5 45 521.5 46 559.0 47 689.5 48 1266.5 49 3857.5 50 6002.0 51 11801.0 52 9427.5 53 996.5 54 738.0 55 682.5 56 547.0 57 450.0 58 433.0 59 459.5 60 453.0 61 388.5 62 357.0 63 370.5 64 351.5 65 294.5 66 264.5 67 259.0 68 227.5 69 188.5 70 143.5 71 106.0 72 106.0 73 91.0 74 65.0 75 54.0 76 52.5 77 44.0 78 33.5 79 30.0 80 27.5 81 24.0 82 17.0 83 11.0 84 9.0 85 5.5 86 5.5 87 7.0 88 5.0 89 2.5 90 2.5 91 2.0 92 1.0 93 2.0 94 4.0 95 11.0 96 17.0 97 20.5 98 90.5 99 4618.0 100 9079.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0064692816940892335 3 0.002156427231363078 4 0.0 5 0.0 6 0.002156427231363078 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001078213615681539 24-25 0.008625708925452311 26-27 0.018329631466586158 28-29 0.01617320423522308 30-31 0.010782136156815388 32-33 0.007547495309770772 34-35 0.005391068078407694 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0064692816940892335 56-57 0.0 58-59 0.005391068078407694 60-61 0.0 62-63 0.0 64-65 0.004312854462726156 66-67 0.002156427231363078 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.012938563388178467 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 46373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.846182045586872 #Duplication Level Percentage of deduplicated Percentage of total 1 76.2697838005586 15.899337976839972 2 11.668563152994723 4.8648998339551035 3 5.079135202234405 3.176417311797813 4 3.175752560256543 2.6480926401138594 5 1.2620254473983654 1.3154206111314772 6 0.7137684907416986 0.8927608737843141 7 0.4448122478535223 0.6490845966402864 8 0.22757835936691836 0.37953119271990166 9 0.13447812144408813 0.2523019860694801 >10 0.7551463742629564 3.2173894291937124 >50 0.13447812144408813 1.9343152265326808 >100 0.08275576704251578 4.810989153171026 >500 0.0 0.0 >1k 0.020688941760628944 6.374398895909257 >5k 0.020688941760628944 31.539904685916376 >10k+ 0.010344470880314472 22.045155586224745 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 10223 22.045155586224745 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 9160 19.752873439285793 No Hit CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 5466 11.787031246630582 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 1844 3.9764518146335153 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 1112 2.3979470812757424 No Hit CATAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 411 0.8862915920902249 No Hit CACTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 362 0.7806266577534341 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 337 0.7267159769693572 No Hit CAAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 290 0.6253638970952925 No Hit CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 283 0.6102689064757509 No Hit ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT 201 0.43344187350397867 RNA PCR Primer, Index 14 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 191 0.41187760119034783 No Hit CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 156 0.3364026480926401 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 91 0.19623487805404005 No Hit TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT 89 0.1919220235913139 No Hit CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 82 0.17682703297177238 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 81 0.1746706057404093 No Hit CAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 80 0.1725141785090462 No Hit TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 69 0.14879347896405234 No Hit CAAAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 67 0.1444806245013262 No Hit CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 62 0.1336984883445108 No Hit TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 60 0.12938563388178465 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT 56 0.12075992495633235 No Hit ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 56 0.12075992495633235 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 53 0.11429064326224311 No Hit CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT 51 0.10997778879951695 No Hit CATATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT 50 0.10782136156815389 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT 50 0.10782136156815389 No Hit AAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT 50 0.10782136156815389 RNA PCR Primer, Index 14 (95% over 21bp) CATATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT 47 0.10135207987406465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004312854462726156 0.0 2 0.0 0.0 0.0 0.004312854462726156 0.0 3 0.0 0.0 0.0 0.004312854462726156 0.0 4 0.0 0.0 0.0 0.010782136156815388 0.0 5 0.0 0.0 0.0 0.017251417850904623 0.0 6 0.0 0.0 0.0 0.017251417850904623 0.0 7 0.0 0.0 0.0 0.0194078450822677 0.0 8 0.0 0.0 0.0 0.02803355400772001 0.0 9 0.0 0.0 0.0 0.02803355400772001 0.0 10 0.0 0.0 0.0 0.03665926293317232 0.0 11 0.0 0.0 0.0 0.04528497185862463 0.0 12 0.0 0.0 0.0 0.053910680784076945 0.0 13 0.0 0.0 0.0 0.06469281694089234 0.0 14 0.0 0.0 0.0 0.0668492441722554 0.0 15 0.0 0.0 0.0 0.07978780756043387 0.0 16 0.0 0.0 0.0 0.07978780756043387 0.0 17 0.0 0.0 0.0 0.07978780756043387 0.0 18 0.0 0.0 0.0 0.0862570892545231 0.0 19 0.0 0.0 0.0 0.12938563388178467 0.0 20 0.0 0.0 0.0 0.13154206111314773 0.0 21 0.0 0.0 0.0 0.14879347896405234 0.0 22 0.0 0.0 0.0 0.1638884695835939 0.0 23 0.0 0.0 0.0 0.1919220235913139 0.0 24 0.0 0.0 0.0 0.2027041597481293 0.0 25 0.0 0.0 0.0 0.2113298686735816 0.0 26 0.0 0.0 0.0 0.2242684320617601 0.0 27 0.0 0.0 0.0 0.25877126776356935 0.0 28 0.0 0.0 0.0 0.28680482177128935 0.0 29 0.0 0.0 0.0 0.35365406594354476 0.0 30 0.0 0.0 0.0 0.36874905656308626 0.0 31 0.0 0.0 0.0 0.42912901904125245 0.0 32 0.0 0.0 0.0 0.49813469044487096 0.0 33 0.0 0.0 0.0 0.6145817609384772 0.0 34 0.0 0.0 0.0 0.8108166389925172 0.0 35 0.0 0.0 0.0 0.9466715545683911 0.0 36 0.0 0.0 0.0 1.2011299678692342 0.0 37 0.0 0.0 0.0 1.690638949388653 0.0 38 0.0 0.0 0.0 2.1564272313630775 0.0 39 0.0 0.0 0.0 2.4906734522243545 0.0 40 0.0 0.0 0.0 2.9133331895715178 0.0 41 0.0 0.0 0.0 3.2928643822914196 0.0 42 0.0 0.0 0.0 3.60123347637634 0.0 43 0.0 0.0 0.0 3.9872339507903307 0.0 44 0.0 0.0 0.0 4.384016561361137 0.0 45 0.0 0.0 0.0 4.780799171931943 0.0 46 0.0 0.0 0.0 5.166799646345934 0.0 47 0.0 0.0 0.0 5.520453712289479 0.0 48 0.0 0.0 0.0 5.930174886248463 0.0 49 0.0 0.0 0.0 6.216979708019753 0.0 50 0.0 0.0 0.0 6.486533111940138 0.0 51 0.0 0.0 0.0 6.769025079248701 0.0 52 0.0 0.0 0.0 7.029952774243633 0.0 53 0.0 0.0 0.0 7.282254760313113 0.0 54 0.0 0.0 0.0 7.56690315485304 0.0 55 0.0 0.0 0.0 7.786858732452074 0.0 56 0.0 0.0 0.0 8.015440018976559 0.0 57 0.0 0.0 0.0 8.194423479179695 0.0 58 0.0 0.0 0.0 8.369094084920105 0.0 59 0.0 0.0 0.0 8.580423953593685 0.0 60 0.0 0.0 0.0 8.761563841028185 0.0 61 0.0 0.0 0.0 8.903888038298147 0.0 62 0.0 0.0 0.0 9.007396545403575 0.0 63 0.0 0.0 0.0 9.134625752053998 0.0 64 0.0 0.0 0.0 9.22088284130852 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGAACG 30 1.0027543E-7 70.0 24 TGCCGAC 25 2.7981841E-6 70.0 45 TCGAATG 20 7.8487436E-5 70.0 40 CTCTCGT 15 0.0022114215 70.0 37 AAGAGGG 15 0.0022114215 70.0 69 GAACGGT 20 7.8487436E-5 70.0 26 TCTATAC 15 0.0022114215 70.0 1 ACTTCTG 15 0.0022114215 70.0 50 ATGTGTA 15 0.0022114215 70.0 8 AACTCGT 15 0.0022114215 70.0 37 CAATACA 15 0.0022114215 70.0 1 AAAAGAG 15 0.0022114215 70.0 67 CGAATGC 20 7.8487436E-5 70.0 41 ATGCCGA 25 2.7981841E-6 70.0 44 CAGATTG 15 0.0022114215 70.0 54 AGACCAC 15 0.0022114215 70.0 17 ACGGTTC 15 0.0022114215 70.0 28 TGTATAA 20 7.8487436E-5 70.0 11 GCCGACT 15 0.0022114215 70.0 46 GACCACG 15 0.0022114215 70.0 18 >>END_MODULE