FastQCFastQC Report
Wed 25 May 2016
SRR1779942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222128
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3204314.425466397752649No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG55582.5021609162284806No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC8790.39571778434056043No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG6500.29262407260678525No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6160.27731758265504575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATC150.002222535270.021
ACGAGTA150.002222535270.049
CGAGTAC200.00694469152.50000450
TACGGCG200.00694469152.50000463
ACGTCTC200.00694469152.50000435
TTACGTC200.00694469152.50000433
ACCCGGC200.00694469152.50000448
GTCGCCG8450.041.00591751
GCCGTAT8450.041.00591754
TGGTCGC8800.040.96590849
TCGGTGG9000.040.83333245
ATCTCGG8750.040.842
CTCGGTG9000.040.44444744
TCTCGGT9000.040.44444743
TCGCCGT8500.040.35294352
GTATCAT8850.040.33898557
CGCCGTA8600.040.290753
CGTATCA8700.040.22988556
GTAGATC8800.040.17045238
TCACCGT350.00125358340.031