FastQCFastQC Report
Wed 25 May 2016
SRR1779942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222128
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99224.466793920622344No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT75303.389937333429374No Hit
CATATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT39111.7606965353309805No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT10390.4677483252899229No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG9120.4105740834113665No Hit
CATATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT6270.2822696823453144No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC5680.255708420370237No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT3570.16071814449326516No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG2800.12605344666138443No Hit
CATATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT2680.12065115609018223No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT2550.11479867463804654No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2340.1053446661384427No Hit
CATAAACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT2230.10039256644817401No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACA150.002219247470.0261223
AAGTCGT150.002223215869.994619
TCGAATG352.9619332E-760.00887740
AGGGGGG18750.058.995470
CATATAC8700.058.73111
TGCCGAC308.361769E-658.3288345
TGACGTA800.056.8706145
GATCTCT252.3877356E-455.99567429
CAGTAGT252.3877356E-455.9956741
CGAGACA700.055.02052723
GCCGTAA2900.054.30615646
ATATACA9650.054.037282
TCGTATG17450.053.96213540
CCGTCTT10000.053.5458747
CTCGTAT18550.053.4041339
GCCGTCT9900.053.3797246
TCTCGTA19550.053.35827638
CCGTAAT3550.053.2353347
CGTATGC17050.053.16305541
ATCTCGT20200.052.8543537