FastQCFastQC Report
Wed 25 May 2016
SRR1779941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371995
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263967.0957942983104605No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG50721.3634591862793854No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC8090.2174760413446417No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG7310.19650801758088146No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6090.16371187784782054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAG200.006948463652.49983
CAGTTAC551.3094541E-744.5452841
TCGCCGT8650.042.4853952
CTCGGTG8700.042.2412244
GCCGTAT8750.041.9998454
TGGTCGC8750.041.9998449
CGGTGGT8750.041.59984246
TCGGTGG8750.041.59984245
GTCGCCG8900.041.29197751
GATCTCG9000.041.2220641
CGTATCA8850.041.12978456
AGATCTC9050.040.99431640
CGCCGTA9150.040.5462953
ATCTCGG9150.040.1637842
TAGATCT9350.040.0533239
GCGTCTA350.001254697739.9998471
GTATCAT9150.039.7812757
TCTCGGT9250.039.72957643
CCGTATC9450.039.2591155
GGCTCTA459.190107E-538.8887371
Produced by FastQC (version 0.11.5)