FastQCFastQC Report
Wed 25 May 2016
SRR1779941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371995
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT99432.6728853882444654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89962.418312074087017No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT55641.4957190284815656No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG17870.4803828008440974No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC16720.4494684068334252No Hit
CACTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC5820.15645371577574968No Hit
CATAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT4690.1260769634000457No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC3730.10027016492157152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.002224666869.992471
AGGGGGG22850.058.9731470
CACGAAT308.374334E-658.3270639
ATCGTAT308.374334E-658.3270639
ATATCGT308.374334E-658.3270637
CGAATGC452.6991984E-854.43858741
GCCGTCT26450.050.939746
TATGCCG27100.050.88013543
CATATAC8950.050.8325231
CCGTCTT26450.050.8073947
CGTCTTC26600.050.65244748
CGTATGC27450.050.48637441
ATGCCGT27350.050.15913844
TCGTATG27800.050.10252840
TGCCGTC27350.050.0311845
CCGACTT352.0860543E-549.99462547
GTATGCC27800.049.7248742
AAGGGGG27200.049.67045669
CTTGAAA26800.049.6215357
CTCGTAT27800.049.59898439