FastQCFastQC Report
Wed 25 May 2016
SRR1779940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339884
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG305878.999246801850044No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG65111.915653575925904No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC10660.3136364171305504No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG7990.23508020383424932No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6570.19330124395381953No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG150.002223849369.997644
TAACCGA150.002223849369.997643
CGCTTTA200.006944725852.50596227
GATCCTA200.00694876852.4982381
AGCTCGA200.00694876852.49823840
CCGTTGT200.00694876852.4982381
GCCGTAT13200.043.74852854
TCGCCGT13250.043.58344352
CGCCGTA13200.043.48338753
CGGTGGT13450.043.19557646
TGGTCGC13500.042.7763449
CGTATCA13550.042.360256
CTCGGTG13550.042.360244
GTATCAT13550.042.360257
TCGGTGG13650.042.3062745
CCGTATC13650.042.04986655
GTCGCCG13800.041.84641651
AGATCTC13750.041.48951340
TCTCGGT13950.041.39645843
TAGATCT13900.041.29357539