FastQCFastQC Report
Wed 25 May 2016
SRR1779940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339884
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117913.4691247602123076No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT111593.2831789669416627No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT65581.9294818232102717No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG22330.6569888550211248No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC18250.5369478998717209No Hit
CATAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT6560.19300702592649255No Hit
CACTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC6460.19006484565322287No Hit
CAAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4360.12827905991455907No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT3940.11592190276682633TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3820.11239128643890268No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3700.10886067011097904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG27600.060.362770
ACGTATG553.6379788E-1157.2646640
CATATAC9550.054.966081
CATCGCA452.6895577E-854.46081526
ATATACA10000.053.892412
AAGGGGG31750.052.14206369
GCCGTCT31600.051.60665546
TATGCCG31950.051.58897843
CCGTCTT31350.051.3484347
CGTCTTC31750.051.26016648
GTATGCC32250.051.10907742
CGTATGC32500.050.93128641
ATGCCGT32550.050.85305444
TCGTATG32750.050.43564240
TGCCGTC32700.050.40574645
CTTGAAA32650.050.38316757
ATCTCGT32100.050.148737
GTCTTCT32300.050.06223749
TCTTCTG32350.049.9848650
CTCGTAT32700.049.97767339
Produced by FastQC (version 0.11.5)