FastQCFastQC Report
Wed 25 May 2016
SRR1779939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110793
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3026427.315805150144868No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG67446.087027158755517No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC12611.1381585479227028No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG8180.7383137923876057No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT7610.6868664987860245No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG1880.16968581047539105No Hit
TATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGT1800.16246513768920418No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG1670.15073154441165054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCAA150.002216007870.0284629
TTAATAC207.898841E-569.996842
ATCTGGC150.002219982769.9968410
TAATCGA150.002219982769.996848
TCTGGCG401.05574145E-861.2472411
CTTAATA252.3821136E-455.9974751
AAGGGGG1750.053.9975670
CCGTCCT200.006924398252.52134332
GCATAAA200.006936782552.4976321
CGTGCGC200.006936782552.497638
GCGCGCG200.006936782552.4976356
CTATACA200.006936782552.497631
ATCAGGC200.006936782552.4976316
TTCGTAT200.006936782552.497632
GTTCGTA200.006936782552.497631
ACCAGTG200.006936782552.4976345
GAAGTTC200.006936782552.4976352
GTCCGAA200.006936782552.4976348
ACATGGA200.006936782552.4976347
CGTCGTG200.006936782552.4976364