FastQCFastQC Report
Wed 25 May 2016
SRR1779939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110793
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1170110.561136533896546No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT1165610.520520249474245No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT64875.855063045499264No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG20641.862933578836208No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC17871.6129177836144883No Hit
CACTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC6510.5875822479759552No Hit
CATAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT4860.4386558717608513No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC4120.37186464848862294No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT3450.311391513904308TruSeq Adapter, Index 2 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2730.24640545882862636No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2710.24460029063207964No Hit
CAAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2450.22113310407697234No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT1440.12997211015136334No Hit
TATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCT1150.10379717130143602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA252.825689E-669.9932337
CTTAAGC207.900859E-569.99322551
GATACGA150.00222043769.99322557
GACGTAA150.00222043769.99322546
GGATACG150.00222043769.99322556
TTCCATG150.00222043769.99322512
TCGTATT150.00222043769.99322540
CTCGAAT207.900859E-569.99322539
GTCTTAA207.900859E-569.99322549
ATATACA9250.065.4531252
AGGGGGG21450.063.95651670
CATATAC9550.063.030551
CTATACA308.335184E-658.327691
TCTCGAA308.335184E-658.3276938
CAAATAC601.8189894E-1258.327691
AGTATCT252.3774055E-456.0198834
CTCGTAA750.055.99458339
TTCCGTC252.3827204E-455.99458345
ATTCCGT252.3827204E-455.99458344
GCTTCTA252.3827204E-455.9945831
Produced by FastQC (version 0.11.5)