FastQCFastQC Report
Wed 25 May 2016
SRR1779938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155502
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2883718.544456019858266No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG63084.056539465730345No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC10610.6823063368959885No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG8160.524752093220666No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6830.4392226466540623No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2160.13890496585252923No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG2080.13376033748762076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTG150.002218971370.0183320
TCGTGCG150.002218971370.018332
GTGCGCG301.016615E-770.018334
AGGTACT150.002221804669.9958267
GTACACC150.002221804669.995825
AACGGCC150.002221804669.9958237
AACGATT150.002221804669.9958268
TGACACA207.9094505E-569.9958125
TTAATAC252.3815047E-456.0146683
CACATGT252.385294E-455.9966628
TGCGCGT252.385294E-455.996665
CAGTGAC252.385294E-455.9966622
TTAACGG252.385294E-455.9966635
TCTATAG252.385294E-455.9966623
CTTTATA452.6777343E-854.4587062
GCCCAGT200.00693360752.5137519
CGATTAA200.00693360752.5137570
GCGTCCC200.00693360752.5137520
GGTCGCA200.006942434752.4968650
TAGGTAC200.006942434752.4968666