FastQCFastQC Report
Wed 25 May 2016
SRR1779938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155502
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112847.256498308703425No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT111127.145888798857893No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT62514.019883988630371RNA PCR Primer, Index 19 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG21371.3742588519761803No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC18361.1806922097464985No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC6900.44372419647335726No Hit
CATAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT5220.33568700081027897RNA PCR Primer, Index 19 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC3740.2405113760594719No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT3720.23922521896824478No Hit
CAAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3490.22443441241913287No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3360.21607439132615658No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC2670.17170197167882087RNA PCR Primer, Index 19 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2170.1395480443981428RNA PCR Primer, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTAGC150.002215854870.0431364
GTTCTGC207.895585E-570.0205924
GTCGCCC207.908182E-569.9980737
TATGCCA150.002221519669.9980749
ATGACGA207.908182E-569.9980744
CCGAAAT207.920796E-569.9755647
GCTCGGA207.920796E-569.9755611
CGACTTC453.8198777E-1062.2205148
AGGGGGG24950.061.88192770
CATATAC9200.060.4680141
ATATACA9400.060.298092
CGTCTAC352.9596958E-759.9983448
CCGACTT352.9662988E-759.9790547
GCCGACT308.347555E-658.33172646
CGTATGA800.056.87343241
GTGCAAC252.3811235E-456.01647623
TTGGTAG252.3811235E-456.01647667
CGCGTAA509.713403E-1056.01647628
AGAGACG252.3811235E-456.01647623
TCGCCCC252.3849134E-455.99845538
Produced by FastQC (version 0.11.5)