FastQCFastQC Report
Wed 25 May 2016
SRR1779937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98663
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3557136.053028997699236No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG75357.637108135775315No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC8790.8909114865755146No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT7800.7905699198281017No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG7090.7186077861001591No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG2190.22196770825942858No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG1630.16520884222048793No Hit
TATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGT1080.10946352736081408No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGC207.892799E-570.000013
TGCGCGT207.892799E-570.000015
CGGGCGC252.8217746E-670.0000112
CGTCGGG252.8217746E-670.000019
AGAGGCG207.892799E-570.0000168
GTGCGCG207.892799E-570.000014
GTCGGGC207.892799E-570.0000110
AGAAGGT150.002218864270.05
GTCTATG150.002218864270.033
GAATAAG150.002218864270.036
TATAGGT150.002218864270.061
ACCCGTC150.002218864270.029
CCGAAGC150.002218864270.029
GCTCGAA150.002218864270.032
GCGTCGG150.002218864270.08
TATGTAC150.002218864270.031
GGCTCGA150.002218864270.031
GACTATG150.002218864270.07
TACTTGT252.3803007E-456.00000416
GTGTGCG252.3803007E-456.0000042
Produced by FastQC (version 0.11.5)