FastQCFastQC Report
Wed 25 May 2016
SRR1779937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98663
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1384214.029575423411005No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1236312.530533229275411No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT81108.219900063853725No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG26312.666653152650943No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC14231.4422833280966523No Hit
CATAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT7150.7246890931757599No Hit
CACTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5420.549344739162604No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5300.5371821250114025No Hit
CAAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4990.5057620384541318No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT4380.44393541651885715No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3780.38312234576284926No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC2810.28480788137396995No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2620.26555040896790083No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT1650.1672359445790215No Hit
AAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT1440.14595136981441878No Hit
CAAAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT1370.1388565115595512No Hit
CATATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT1340.1358158580217508No Hit
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1280.12973455094615002No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC1230.12466679504981604No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT1160.11757193679494847No Hit
CAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1140.11554483443641486No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1120.11351773207788128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGA150.00221535870.02788534
AAACTCG650.069.992436
CTTAAGC150.0022198269.9923951
AGCAACA150.0022198269.992392
TCTTAAG150.0022198269.9923950
CGTCTTA301.0153599E-769.9923948
TAACGTA150.0022198269.9923938
CACGAAT150.0022198269.9923939
GTCAGTA150.0022198269.9923956
CCGTACG150.0022198269.9923952
TAACCGC150.0022198269.9923961
CGTACGC150.0022198269.9923953
ATAACGT150.0022198269.9923937
CCTGGCC150.0022198269.9923947
GCCAGTG150.0022198269.9923913
GTCTTCA455.456968E-1269.9923949
CCAGTGC150.0022198269.9923914
AAGCTTG150.0022198269.9923954
GTACGCC150.0022198269.9923954
ATATACA8950.066.473222
Produced by FastQC (version 0.11.5)