FastQCFastQC Report
Wed 25 May 2016
SRR1779936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341332
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293178.588998394524978No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG56811.664361970163946No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC9830.287989406208618No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG6500.19043043136887253No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT6030.17666084633143098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCG308.364514E-658.3368765
CGGTTCG200.00695022952.49549514
TCTGGAT200.00695022952.49549510
GCCGTAT10100.044.35912754
TCGCCGT10200.044.2609152
TGGTCGC10400.043.74624649
TCGGTGG10500.043.66291845
CGTATCA10350.043.6258456
GATCTCG10550.043.46235341
CTCGGTG10550.043.45598644
GTCGCCG10500.043.32961351
CGGTGGT10600.043.25100746
TCTCGGT10750.042.97935543
GTATCAT10350.042.94946757
ATCTCGG10750.042.6537542
GTAGATC10750.042.3281538
AGATCTC10900.042.06677240
GTGGTCG10950.041.86855348
TAGATCT10900.041.74565539
CGCCGTA10850.041.2928353