FastQCFastQC Report
Wed 25 May 2016
SRR1779936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341332
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT115443.3820444611111764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98772.8936636471236215No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT61311.7961984226500884No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG21640.6339868515111386No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC17870.5235372013171926No Hit
CACTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC6160.18046945495880845No Hit
CATAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4650.13623100090234727No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3980.11660201797663274No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.00222306270.0039539
ATACCGT150.00222306270.003956
TACCGTG150.00222306270.003957
CACGTAT352.9685725E-760.0033939
AGGGGGG22350.057.78849870
ACGTATG407.4658055E-752.50296440
AACTCGT200.00694631952.50296437
CGATACC200.006950345452.49527431
GCCGTCA1150.051.74205446
CATATAC9200.048.3179471
TATGCCG28450.047.85859343
CGTATGC28450.047.61253441
ATGCCGT28550.047.44576344
TGCCGTC28450.047.24344345
CTCGTAT29000.047.1923239
TCGTATG29150.047.1896340
GTATGCC29100.047.1504342
GCCGTCT27350.047.095946
CCGTCTT27150.047.0560747
CGTCTTC27350.046.9610448
Produced by FastQC (version 0.11.5)