FastQCFastQC Report
Wed 25 May 2016
SRR1779935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184491
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3475618.838859348152486No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG72483.9286469258663024No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC11690.6336352450797058No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT7900.42820516989988666No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG7800.422784851293559No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG2400.13008764655186433No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2050.11111653142971743No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCAG207.909334E-570.0029952
GCTTTTT207.909334E-570.0029928
GCTGCGA150.0022215670.0029824
ATACACG150.002223950869.984014
CTCACCG252.3852565E-456.00238817
GGCTTTT252.3852565E-456.00238827
TAACCGG252.3852565E-456.00238822
TAAGGAC252.3852565E-456.00238819
ACGGTGC252.3852565E-456.0023888
CATGCGG200.006934216752.5164842
ACCGTCG200.006934216752.5164844
ATGCCAT200.006934216752.5164843
ACTTCGT200.0069416652.50224328
CCGTCGG200.0069416652.50224345
AAACGAG200.0069416652.50224311
AACGAGC200.0069416652.50224312
TTCGCGG200.0069416652.5022438
GTGCGCA200.0069416652.50224323
TAATACG200.00694910952.488014
GGTCACG200.00694910952.4880158