FastQCFastQC Report
Wed 25 May 2016
SRR1779935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184491
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT133927.258890677594029No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127286.89898152213387No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT75654.10047102568689No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG26931.4596918006840442No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC18090.9805356358846773No Hit
CATAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT7300.3956832582619207No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC7210.3908049715162257No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC5890.31925676591270036No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT5770.3127523835851071RNA PCR Primer, Index 19 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4670.2531288789155027No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT4310.23361573193272303No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC3390.18374880075450833No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2940.15935736702603379No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT2020.10949043584781915RNA PCR Primer, Index 19 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.8888595E-570.0396126
CTCGTTT150.00221695470.0396126
GCTCGTT150.002219341270.02061525
CGTTATA150.002221730370.0016252
GGTGTCG150.002224121269.982669
ATATCGT150.002224121269.9826637
GTGTCGA150.002224121269.9826610
TTCGGTG207.920726E-569.9826535
AGGGGGG28250.063.04631470
CATATAC9000.061.0569761
CGTATGA700.059.9851341
AATATCG308.364626E-658.3188836
ATATACA9900.056.566972
CGGGGTG252.3790964E-456.03168531
CGCTTTT252.3790964E-456.03168530
TCCGAGA509.749783E-1056.001313
CCGTCAA252.3886836E-455.98612247
AAGGGGG32450.054.56274469
AACGGCA200.0069273152.52971329
TCGTGAC200.0069273152.52971332
Produced by FastQC (version 0.11.5)