FastQCFastQC Report
Wed 25 May 2016
SRR1779934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338867
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3746711.056550209964382No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG9060.2673615312202132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCG200.006948639652.49844716
GTTATGC305.8743573E-446.665291
GCATAGG305.8743573E-446.665291
GCGCGTC350.00125469839.9988176
GCTATAC350.00125469839.9988173
GTATACG350.00125469839.9988172
CGCGTCG350.00125469839.9988177
TACCACG459.189766E-538.8877420
TGTGCGC459.189766E-538.887743
CCCTTGG556.6865923E-638.180691
CCACGCT556.6865923E-638.1806922
CACGCTA601.2130387E-535.00413523
TGGCGGC501.7082402E-434.99896619
CAACCGT400.002417384634.9989669
GCCATAG400.002417384634.9989661
GGGAACC400.002417384634.99896643
GCCGTAT1500.032.66570354
TCCGATA552.9869634E-431.82193829
GCGTCGG552.989551E-431.8172428
AACCGTA450.004304838431.11019310
Produced by FastQC (version 0.11.5)