FastQCFastQC Report
Wed 25 May 2016
SRR1779934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338867
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT228176.73332015215409No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG37871.1175475924182645No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC26870.7929364617976964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18430.5438711943033697No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT15690.46301351267606466Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC8270.24404855002109974No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC5150.15197702933599316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGT207.921557E-569.9982215
AGGGGGG26800.058.63657870
CTTTATA1900.055.261762
CGTCTAC200.00694852452.4986748
GCCGTCT36600.047.1436246
CCGTCTT36450.047.04956447
CGTCTTC36850.047.01373748
AGCCGTC305.8742345E-446.6654945
ATCGTAT604.9240043E-946.6654939
CGGTATG305.8742345E-446.6654943
TATACGG305.8742345E-446.665492
TATGCCG37800.046.4803143
TGCCGTC37850.046.14150245
CGTATGC38400.046.1186341
TCTTCTG37200.046.10098350
ATGCCGT38150.046.0538844
GTATGCC38350.045.90496442
TCGTATG39300.045.7749240
CATCTCG38850.045.76460636
CTCGTAT38900.045.70578439