FastQCFastQC Report
Wed 25 May 2016
SRR1779933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191068
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2604013.628655766533381No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG4860.2543597043984341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA207.911734E-570.0000153
TACGGGT150.002222058670.069
GTAGCGA150.002222058670.022
GCGAAGT308.353491E-658.33333232
GGCCCTA308.353491E-658.3333321
GATCCTT200.00694321252.5000041
TTGTCTA200.00694321252.50000451
CGAGGCA200.00694321252.50000426
ATCGAGG200.00694321252.50000424
GATGTAT506.165101E-849.00000424
ATTAGAG305.867036E-446.6666644
CGAGAAT551.3049248E-744.54545614
CGTGCGA503.4656168E-642.00000410
ATCCTCG350.00125314340.034
CGTATCG350.00125314340.013
GATCATA350.00125314340.01
TGCGCCT350.00125314340.051
GTATCGG350.00125314340.014
ACATGTT350.00125314340.030
CTAATCG350.00125314340.027
Produced by FastQC (version 0.11.5)