FastQCFastQC Report
Wed 25 May 2016
SRR1779933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191068
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT153828.050536981598174No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG27601.4445119015219712No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC15540.8133230054221533No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT10170.5322712332782046RNA PCR Primer, Index 34 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9990.5228505034856701No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC5910.3093139615215525No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3440.18004061381288336No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT3130.16381602361462935No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT2300.1203759917934976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAAC150.00222288269.99345451
CGTCTAC207.915399E-569.99345448
AGGGGGG18450.056.7155670
CACGTAG252.3839927E-456.00942226
ATAGACT252.3870793E-455.9947628
CCGCAGA200.00693859252.50883531
CAGTAGT200.006945780552.495091
GCACTTA200.006945780552.495091
ATCGTAT552.2719178E-950.90433539
GTCTTCA850.049.40714649
CTTTATA1100.047.7228132
CGTGATA305.862166E-446.6745229
AAGCCGT305.869738E-446.66230444
CTGCATG1200.046.66230454
CGTACAC305.869738E-446.6623041
GCATGAA1750.045.995756
TCTTTAT1150.045.6479071
GCCGTCA1000.045.49574746
GCCGTCT26200.044.74772646
CCGTCTT25950.044.5045147
Produced by FastQC (version 0.11.5)