FastQCFastQC Report
Wed 25 May 2016
SRR1779932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87052
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2777231.902770757708037No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG6060.6961356430639158No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1370.15737719983458162No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT150.00221798470.034
GGTGTAA200.006930579452.51
CCGTACC200.006930579452.545
GGGCCGC200.006930579452.525
GGTCTCG200.006930579452.541
TCAAGTG200.006930579452.525
GGCTAAT200.006930579452.516
TCTGGCG305.849343E-446.66666411
ACTATCT350.001249386940.035
CGTATCA1007.2759576E-1238.556
CCGTATC1007.2759576E-1238.555
TCGGTGG1007.2759576E-1238.545
GTGGTCG1100.038.18181648
TCGCCGT1051.2732926E-1136.66666852
GTCGCCG1051.2732926E-1136.66666851
CTCGGTG1051.2732926E-1136.66666844
GTATCAT1051.2732926E-1136.66666857
CGCCGTA1051.2732926E-1136.66666853
TGGTCGC1051.2732926E-1136.66666849
GTAGATC1102.3646862E-1135.038