FastQCFastQC Report
Wed 25 May 2016
SRR1779932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87052
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1639018.827827045903597No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG27713.183154895924275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC16671.9149473877682306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12861.4772779488122043No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT11881.3647015576896566TruSeq Adapter, Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC5930.6812020401599044No Hit
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4470.5134861921610072No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3660.420438358682167No Hit
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT2250.2584662041078895No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT1910.2194090888204751No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1650.1895418830124523No Hit
TTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1520.17460828010844093No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGACGT1280.14703855167026605No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG1270.1458898129853421No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC1170.13440242613610257No Hit
CTTATACACATCTCCGAGCCCACGAGACACAGGCTAATCTCGTATGCCGT1000.11487386849239535No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCAGT910.10453522032807976No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCA150.00221798470.028
ACCGGAT150.00221798470.029
AATCACG150.00221798470.036
ACTCTCG150.00221798470.036
CGTCTAC252.8189806E-670.048
CCGTCTA207.8875884E-570.047
TATCCGA207.8875884E-570.011
TCACGTA150.00221798470.038
TCGAATG352.9438888E-760.040
AGGGGGG21950.059.79498770
AGGACTT308.315479E-658.3333325
TCTCGAA308.315479E-658.33333238
TCTTTAT900.058.333331
ACAACGG252.3787386E-455.99999627
GGACTTT252.3787386E-455.9999966
GACAACG252.3787386E-455.99999626
ATATCCG252.3787386E-455.99999610
CGAATGC252.3787386E-455.99999641
GTGCAAA200.006930579452.558
TTAGGAC200.006930579452.53
Produced by FastQC (version 0.11.5)