FastQCFastQC Report
Wed 25 May 2016
SRR1779931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290199
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235538.116154776549884No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG4100.14128236141406414No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC207.919322E-569.9987951
GGTTGGA150.00222337669.9987951
TACCCGC407.465678E-752.49909611
GGGACTA200.006947299552.4990961
GATATTA200.006947299552.4990961
GATCATA352.0837959E-549.9991341
AGGTGTC305.8676105E-446.6739115
GCCGTAT1050.046.66586354
TCGGTGG1100.044.5446945
TTCTGTT404.5867426E-543.7492538
CTCGGTG1150.042.6079644
CGTGATA503.4722798E-641.99927524
GCTTCTA503.4722798E-641.9992751
CTACCCG503.4722798E-641.99927510
AGATCTC1200.040.8326340
TCGCCGT1300.040.3839252
CGTATCA1300.040.3839256
GATGTAT350.001254324739.9993124
CGTGCGA350.001254324739.9993110
CCGTGCG350.001254324739.999319
Produced by FastQC (version 0.11.5)