FastQCFastQC Report
Wed 25 May 2016
SRR1779931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290199
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT130064.481752176954434No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG24750.8528630353653872No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC17050.5875278688072668No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT9830.3387330762683538RNA PCR Primer, Index 14 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8520.29359163884093326No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC4180.1440390904172654No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC2980.10268815536924661No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA150.002222745770.0037948
AACGACG150.002222745770.0037970
ACGTATG207.923282E-569.9917340
TGCCGTA207.923282E-569.9917345
AGTCTTC352.9670628E-760.0032548
CACGTAT252.3894438E-455.9933839
AGGGGGG16350.053.3056470
CTGCGCT200.00694533752.50284652
CTTGAGT200.00695007352.49379731
CTTTATA707.2759576E-1250.0113262
GTTACCA352.0850399E-549.9940931
ACACATT305.8705296E-446.6691976
TCAGCAT305.8705296E-446.66919753
CTAAGCG305.8755203E-446.6611545
ATCGTAT451.6827744E-646.6611539
ATATCGT451.6827744E-646.6611537
TTCCCAA305.8755203E-446.6611532
AATCTCG305.8755203E-446.6611536
CGGAGAG305.8755203E-446.6611536
CGTATGC23500.045.27124441