FastQCFastQC Report
Wed 25 May 2016
SRR1779930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140929
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2702319.174903674900126No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG5930.4207792576403721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGAG207.904537E-570.07
GCCGTAT1150.057.8260954
ACCAGCA252.3838188E-456.00000413
TGGTCGC1250.056.00000449
CGGTGGT1300.053.84615746
GCGAAGT200.006939448452.532
GATCCTA200.006939448452.51
GTTGTCA200.006939448452.558
GCTAGAG200.006939448452.537
CGCAAAG200.006939448452.512
TCGGTGG1300.051.1538545
ATCTCGG1400.050.00000442
TCGCCGT1350.049.25926252
TCTCGGT1350.049.25926243
GTCGCCG1450.048.2758651
TAGATCT1450.048.2758639
AGATCTC1400.047.50000440
GATCTCG1400.047.50000441
TCTGGCG305.861761E-446.66666411
CGAAGTT305.861761E-446.66666433
Produced by FastQC (version 0.11.5)