FastQCFastQC Report
Wed 25 May 2016
SRR1779930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140929
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT72535.1465631630111615No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT17541.2445983438469017No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG12130.860717098680896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11420.8103371201101264No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT5600.39736321126240876No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC5040.35762689013616783No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG4220.2994415627727437No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT3870.27460636206884315No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT3330.23628919526853948No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT3100.21996892052026196No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT2640.18732837102370697No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT2360.16746021046058654No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT2330.16533147897168077No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA1920.1362388152899687No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCG1770.12559515784543993No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCCT1640.11637065472684827No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC1490.10572699728231946No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCAT1450.10288868863044512No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCG1410.10005037997857077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGAA252.8280683E-670.0000145
CCTATGC150.002220844470.041
GACGAAA453.8198777E-1062.22221846
CGCGGAA352.957131E-760.00000431
CGGCAAC553.45608E-1157.2727333
AGGGGGG12100.055.82644770
AATGCCG452.6808266E-854.44444343
GCCGTAA3050.053.93442546
CATCAGC1500.053.66666851
TGCCGTA4450.052.6966345
TCCGAAA200.006939448452.547
ATCGTAT200.006939448452.539
TACGCGG200.006939448452.529
GCCGAAA407.437138E-752.546
AGTTGGC200.006939448452.536
CGGATAA200.006939448452.551
CTACGCG200.006939448452.528
ATGACAC200.006939448452.553
TACAGCC200.006939448452.54
ATGCCGG200.006939448452.544
Produced by FastQC (version 0.11.5)