FastQCFastQC Report
Wed 25 May 2016
SRR1779929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250294
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174176.958616666799844No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAGT252.3874117E-456.0000041
CACGCGA200.006945715852.558
ACTGGCG200.006945715852.565
CGGTATG305.870541E-446.66666843
TCGCCGT659.9717E-943.07692752
GCCGTAT701.9179424E-840.054
TGGTCGC801.4151738E-939.37549
CTAGAGC501.7066696E-435.0000044
CGAGAAT501.7066696E-435.00000414
TGGTCTC501.7066696E-435.00000441
GGCTCTA400.00241583135.01
TCGGTGG851.05934305E-732.94117745
GATCTCG751.4856669E-632.66666841
GATCCTA652.0974896E-532.3076931
TAGAGCC552.986816E-431.818185
GTAAAGC552.986816E-431.8181816
AGTCCCC450.004302086331.11110954
TTTCGTC450.004302086331.11110940
ATCTCGG802.4584351E-630.62542
CTCGGTG802.4584351E-630.62544
Produced by FastQC (version 0.11.5)