FastQCFastQC Report
Wed 25 May 2016
SRR1779929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250294
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT90533.616946470950163No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG18620.7439251440306199No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC9470.3783550544559598No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT8290.33121049645616757Illumina PCR Primer Index 11 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5510.22014111404987735No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT4840.19337259382965633No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3910.15621628964337939No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC3870.15461816903321696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC252.3884898E-455.994893
AGGGGGG12700.052.08185670
CTTTATA800.048.130222
CACGCTA305.855847E-446.6903923
GCAGCGT305.867401E-446.6717341
AGCGTCA305.8731844E-446.6624073
GCCGTCT16050.043.82777446
CCGATAA404.5707202E-543.7722422
CAGAGTA404.5815228E-543.754741
CGTCTTC16100.043.6916648
TCGTATG17500.043.39603440
CCGTCTT16050.043.39167847
CTCGTAT17350.043.36779439
TATGCCG17100.043.18319343
TCTCGTA17500.042.9960738
TCTTCTG16450.042.97479650
CGTATGC17350.042.96437541
ATGCCGT17300.042.88625344
GTATGCC17350.042.76266542
CTTCTGC16700.042.75058751