FastQCFastQC Report
Wed 25 May 2016
SRR1779928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263018
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262409.976503509265527No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG5860.2227984396505182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGG150.00222272870.002098
GCCTTTA150.00222440569.9887851
ATCTAGC150.00222440569.9887853
GATCTTA150.00222440569.9887851
GGTCTAA150.00222440569.9887851
GGTATCA252.3895277E-455.9910281
GACCGCG200.00694528552.50156827
TTAATAC200.006950510752.491593
TGAACAC200.006950510752.491595
GAATGGT305.8757304E-446.6591937
GCTTATC404.5894387E-543.742991
GGCCGGC350.001252646940.00880419
AAGTACC350.001253819140.00119855
GAGGTTC350.001254992339.9935935
TGTATAA459.191226E-538.882662
TCTTCAG400.002413445935.007717
AAGCGCA400.00241569935.00104549
TAAGCGC400.00241569935.00104548
GCCGTAT1700.035.00104554
GGGACGC400.00241795434.99439235
Produced by FastQC (version 0.11.5)